RGD Reference Report - Immunohistochemical characterization of glomerular PDGF B-chain and PDGF beta-receptor expression in diabetic rats.

General

Immunohistochemical characterization of glomerular PDGF B-chain and PDGF beta-receptor expression in diabetic rats.
Authors:	Nakagawa, H Sasahara, M Haneda, M Koya, D Hazama, F Kikkawa, R
Citation:	Nakagawa H, etal., Diabetes Res Clin Pract. 2000 May;48(2):87-98.
RGD ID:	2311654
Pubmed:	PMID:10802145 (View Abstract at PubMed)
Platelet-derived growth factor (PDGF) was found to contribute to the pathophysiological process in the development and progression of glomerulosclerosis characterized by mesangial cell proliferation and accumulation of extracellular matrix. To examine the role of PDGF in the development of diabetic nephropathy, we conducted immunohistochemical analysis for PDGF B-chain (PDGF-B) and PDGF beta-receptor (PDGFR-beta) in the glomeruli of streptozotocin-induced diabetic rats. At 2, 4, and 12 weeks after the onset of diabetes, the expression of PDGF-B in glomeruli of diabetic rats was increased significantly as compared to control or diabetic rats treated with insulin. Similar changes were observed on PDGFR-beta immunostaining. The immunostaining of mirror sections revealed the existence of PDGF-B or PDGFR-beta not only in mesangial cells but also in visceral epithelial cells. Glomerular volume was significantly increased in diabetes. This early glomerular abnormality was prevented by an inhibition of PDGF system with trapidil as well as by the treatment of insulin. Our results suggest that the activation of the PDGF system in glomerular cells might play an important role in the development of early glomerular lesion in diabetes.

RGD Manual Disease Annotations Click to see Annotation Detail View

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
Experimental Diabetes Mellitus		ISO	Pdgfrb (Rattus norvegicus)	2311654; 2311654	protein:increased expression:glomerulus	RGD
Experimental Diabetes Mellitus		IEP		2311654	protein:increased expression:glomerulus	RGD

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Cellular Component

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
cytoplasm	located_in	IDA		2311654; 2311654	PMID:10802145	UniProt
plasma membrane	located_in	IDA		2311654	PMID:10802145	UniProt

Objects Annotated

Genes (Rattus norvegicus)

Pdgfb (platelet derived growth factor subunit B)

Pdgfrb (platelet derived growth factor receptor beta)

Genes (Mus musculus)

Pdgfrb (platelet derived growth factor receptor, beta polypeptide)

Genes (Homo sapiens)

PDGFRB (platelet derived growth factor receptor beta)

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Immunohistochemical characterization of glomerular PDGF B-chain and PDGF beta-receptor expression in diabetic rats.