RGD Reference Report - Novel interaction of the dopamine D2 receptor and the Ca2+ binding protein S100B: role in D2 receptor function. - Rat Genome Database

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Novel interaction of the dopamine D2 receptor and the Ca2+ binding protein S100B: role in D2 receptor function.

Authors: Liu, Y  Buck, DC  Neve, KA 
Citation: Liu Y, etal., Mol Pharmacol. 2008 Aug;74(2):371-8. Epub 2008 Apr 29.
RGD ID: 2311563
Pubmed: PMID:18445708   (View Abstract at PubMed)
PMCID: PMC2574776   (View Article at PubMed Central)
DOI: DOI:10.1124/mol.108.044925   (Journal Full-text)

S100B is a calcium-binding protein with both extracellular and intracellular regulatory activities in the mammalian brain. We have identified a novel interaction between S100B and the dopamine D(2) receptor. Our results also suggest that the binding of S100B to the dopamine D(2) receptor enhances receptor signaling. This conclusion is based on the following observations: 1) S100B and the third cytoplasmic loop of the dopamine D(2) receptor interact in a bacterial two-hybrid system and in a poly-histidine pull-down assay; 2) immunoprecipitation of the D(2) receptor also precipitates FLAG-S100B from human embryonic kidney 293 cell homogenates and endogenous S100B from rat neostriatal homogenates; 3) S100B immunoreactivity was detected in cultured neostriatal neurons expressing the D(2) receptor; 4) a putative S100B binding motif is located at residues 233 to 240 of the D(2) receptor, toward the amino terminus of the third cytoplasmic loop. D(3)-IC3, which does not bind S100B, does not contain this motif; and 5) coexpression of S100B in D(2) receptor-expressing 293 cells selectively increased D(2) receptor stimulation of extracellular signal-regulated kinases and inhibition of adenylate cyclase.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIS100b (Rattus norvegicus)2311563 RGD 
signaling receptor binding  IPIDrd2 (Rattus norvegicus)2311563 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Drd2  (dopamine receptor D2)
S100b  (S100 calcium binding protein B)


Additional Information