RGD Reference Report - Characterization of diabetic nephropathy by urinary proteomic analysis: identification of a processed ubiquitin form as a differentially excreted protein in diabetic nephropathy patients. - Rat Genome Database

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Characterization of diabetic nephropathy by urinary proteomic analysis: identification of a processed ubiquitin form as a differentially excreted protein in diabetic nephropathy patients.

Authors: Dihazi, H  Muller, GA  Lindner, S  Meyer, M  Asif, AR  Oellerich, M  Strutz, F 
Citation: Dihazi H, etal., Clin Chem. 2007 Sep;53(9):1636-45. Epub 2007 Jul 18.
RGD ID: 2311211
Pubmed: PMID:17634209   (View Abstract at PubMed)
DOI: DOI:10.1373/clinchem.2007.088260   (Journal Full-text)

BACKGROUND: Identification of markers for prediction of the clinical course of diabetic nephropathy remains a major challenge in disease management. We established a proteomics approach for identification of diabetic nephropathy-related biomarkers in urine. METHODS: We used SELDI-TOF mass spectrometry and SAX2 protein arrays to compare protein profiles from urine of 4 defined patient groups. Samples from patients with type 2 diabetes (DM; n = 45) without nephropathy and without microalbuminuria (DM-WNP), patients with DM with macro- or microalbuminuria (DM-NP; n = 38), patients with proteinuria due to nondiabetic renal disease (n = 34), and healthy controls (n = 45) were analyzed. Anionic exchange, reversed-phase fractionation, gel electrophoresis, and mass spectrometry were used to isolate and identify proteins with high discriminatory power. RESULTS: A protein with m/z 6188 (P <0.0000004) was strongly released in the urine of healthy controls, patients with proteinuria due to nondiabetic disease, and DM-WNP in contrast to DM-NP patients. An m/z 14 766 protein (P <0.00008) was selectively excreted in the urine of DM-NP patients, whereas the protein with m/z 11 774 (P <0.000004) was significantly excreted by patients with proteinuria and DM-NP. The m/z 11 774 and m/z 14 766 mass peaks were identified as beta(2)-microglobulin and UbA52, a ubiquitin ribosomal fusion protein, respectively. The protein with m/z 6188 was identified as a processed form of ubiquitin. CONCLUSION: The release of high amounts of UbA52 in urine of DM-NP patients could serve as a diagnostic marker, whereas the lack of the short form of ubiquitin raises interesting questions about the pathophysiology.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Diabetic Nephropathies  IEP 2311211associated with Diabetes Mellitus more ...RGD 
Diabetic Nephropathies  ISOUBA52 (Homo sapiens)2311211; 2311211associated with Diabetes Mellitus more ...RGD 
proteinuria  IEP 2311211protein:increased expression:urineRGD 
proteinuria  ISOB2M (Homo sapiens)2311211; 2311211protein:increased expression:urineRGD 

Objects Annotated

Genes (Rattus norvegicus)
B2m  (beta-2 microglobulin)
Uba52  (ubiquitin A-52 residue ribosomal protein fusion product 1)

Genes (Mus musculus)
B2m  (beta-2 microglobulin)
Uba52  (ubiquitin A-52 residue ribosomal protein fusion product 1)

Genes (Homo sapiens)
B2M  (beta-2-microglobulin)
UBA52  (ubiquitin A-52 residue ribosomal protein fusion product 1)


Additional Information