RGD Reference Report - Accumulation of apolipoproteins in the regenerating and remyelinating mammalian peripheral nerve. Identification of apolipoprotein D, apolipoprotein A-IV, apolipoprotein E, and apolipoprotein A-I. - Rat Genome Database

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Accumulation of apolipoproteins in the regenerating and remyelinating mammalian peripheral nerve. Identification of apolipoprotein D, apolipoprotein A-IV, apolipoprotein E, and apolipoprotein A-I.

Authors: Boyles, JK  Notterpek, LM  Anderson, LJ 
Citation: Boyles JK, etal., J Biol Chem. 1990 Oct 15;265(29):17805-15.
RGD ID: 2311210
Pubmed: PMID:2120218   (View Abstract at PubMed)

In this report, we have identified two apolipoproteins (apo), apoD and apoA-IV, that, together with the previously identified apoA-I and apoE, accumulate in the regenerating peripheral nerve. These four apolipoproteins were identified in regenerating rat sciatic nerves by their molecular weights, their isoelectric points, and their recognition by specific antibodies. Antibodies were also used to document the changing concentrations of these apolipoproteins in homogenates of regenerating sciatic nerves collected 1 day to 6 weeks after a denervating crush injury. By 3 weeks after injury, at their peak accumulation, apoA-IV and apoA-I had increased 14- and 26-fold, respectively, relative to their concentrations in the normal nerve. Apolipoproteins D and E, in contrast, increased over 500- and 250-fold, respectively, by 3 weeks. These same apolipoproteins also accumulated in the regenerating sciatic nerves of two other species, the rabbit and the marmoset monkey. Immunocytochemistry showed that apoD was produced by astrocytes and oligodendrocytes in the normal central nervous system, and by neurolemmal or fibroblastic cells in the normal peripheral nervous system. Metabolic labeling of both apoD and apoE by [35S]methionine during an in vitro incubation of regenerating rat sciatic nerve segments confirmed that these apolipoproteins are synthesized by the nerve. Neither apoA-IV nor apoA-I was metabolically labeled, however, suggesting that they enter the nerve from the plasma. The results from this study provide evidence that several different apolipoproteins from various sources may play a role in lipid transport within neural tissues.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Peripheral Nerve Injuries  ISOApoa4 (Rattus norvegicus)2311210; 2311210protein:increased expression:sciatic nerve (rat)RGD 
Peripheral Nerve Injuries  IEP 2311210protein:increased expression:sciatic nerve (rat)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
peripheral nervous system axon regeneration  IEP 2311210; 2311210; 2311210; 2311210 RGD 
response to axon injury involved_inIEP 2311210PMID:2120218UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Apoa1  (apolipoprotein A1)
Apoa4  (apolipoprotein A4)
Apod  (apolipoprotein D)
Apoe  (apolipoprotein E)

Genes (Mus musculus)
Apoa4  (apolipoprotein A-IV)

Genes (Homo sapiens)
APOA4  (apolipoprotein A4)


Additional Information