RGD Reference Report - Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets. - Rat Genome Database

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Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets.

Authors: Iezzi, M  Eliasson, L  Fukuda, M  Wollheim, CB 
Citation: Iezzi M, etal., FEBS Lett. 2005 Sep 26;579(23):5241-6.
RGD ID: 2311147
Pubmed: PMID:16165130   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2005.08.047   (Journal Full-text)

Synaptotagmins (Syts) are involved in Ca(2+)-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet cells had no effect on the expression of Syt 5, Syt 7 and Syt 3 isoforms. The latter was localized at the plasma membrane of pancreatic polypeptide cells. Insulin release in response to glucose or tolbutamide was strongly inhibited in Syt 9 deficient islets, whereas exocytosis potentiated by raising cAMP levels, was unaltered. Thus, Syt 9 may act as Ca(2+) sensor for beta-cell secretion.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
regulation of insulin secretion  IMP 2311147 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
insulin secretion pathway   ISOSyt9 (Rattus norvegicus)2311147; 2311147 RGD 
insulin secretion pathway   IMP 2311147 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Syt9  (synaptotagmin 9)

Genes (Mus musculus)
Syt9  (synaptotagmin IX)

Genes (Homo sapiens)
SYT9  (synaptotagmin 9)


Additional Information