RGD Reference Report - Interleukin (IL)-10 inhibits RANTES-, tumour necrosis factor (TNF)- and nerve growth factor (NGF)-induced mast cell migratory response but is not a mast cell chemoattractant. - Rat Genome Database

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Interleukin (IL)-10 inhibits RANTES-, tumour necrosis factor (TNF)- and nerve growth factor (NGF)-induced mast cell migratory response but is not a mast cell chemoattractant.

Authors: Pietrzak, A  Misiak-Tloczek, A  Brzezinska-Blaszczyk, E 
Citation: Pietrzak A, etal., Immunol Lett. 2009 Mar 24;123(1):46-51. Epub 2009 Feb 14.
RGD ID: 2311043
Pubmed: PMID:19428551   (View Abstract at PubMed)
DOI: DOI:10.1016/j.imlet.2009.02.003   (Journal Full-text)

Interleukin (IL)-10 is an important immunoregulatory cytokine with multiple biologic effects on different cell types. This cytokine also affects mast cell development, survival and activity. Mast cells are well known for their role in diverse pathophysiological processes including inflammatory events. Mast cell number in tissues is high and relatively constant. However, it is well established that these cells accumulate at the sites of inflammation in response to chemoattractants, e.g. RANTES, tumour necrosis factor (TNF) and nerve growth factor (NGF). In the present study, we examined whether IL-10 influenced RANTES-, TNF- and NGF-induced rat peritoneal mast cell migration. We also studied whether IL-10 could act as mast cell chemoattractant. We provided evidence, for the first time ever, that IL-10 influenced mature mast cell migration, i.e. it strongly decreased RANTES-induced mast cell migration and completely inhibited mast cell migratory response to TNF and NGF. The effective concentration of IL-10 that inhibited RANTES-, TNF- and NGF-induced mast cell migratory response was in the nanomolar range. The inhibitory effect of IL-10 on cytokine-stimulated mast cell migration was specific, as it was completely blocked by anti-IL-10R antibodies, and STAT3-dependent. In addition, our results have shown that IL-10 was not a mast cell chemoattractant. Thus, our findings clearly demonstrated that IL-10 may affect mast cell number within tissue by inhibiting local mast cell accumulation stimulated by chemotactic factors.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
interleukin-10 binding  IMP 2311043 RGD 
interleukin-10 receptor activity  IMP 2311043 RGD 
interleukin-10 receptor binding  IMP 2311043 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Objects Annotated

Genes (Rattus norvegicus)
Il10  (interleukin 10)
Il10ra  (interleukin 10 receptor subunit alpha)
Stat3  (signal transducer and activator of transcription 3)

Genes (Mus musculus)
Il10  (interleukin 10)
Il10ra  (interleukin 10 receptor, alpha)
Stat3  (signal transducer and activator of transcription 3)

Genes (Homo sapiens)
IL10  (interleukin 10)
IL10RA  (interleukin 10 receptor subunit alpha)
STAT3  (signal transducer and activator of transcription 3)


Additional Information