RGD Reference Report - Ca(V)3 T-type calcium channel isoforms differentially distribute to somatic and dendritic compartments in rat central neurons. - Rat Genome Database

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Ca(V)3 T-type calcium channel isoforms differentially distribute to somatic and dendritic compartments in rat central neurons.

Authors: McKay, BE  McRory, JE  Molineux, ML  Hamid, J  Snutch, TP  Zamponi, GW  Turner, RW 
Citation: McKay BE, etal., Eur J Neurosci. 2006 Nov;24(9):2581-94.
RGD ID: 2308880
Pubmed: PMID:17100846   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1460-9568.2006.05136.x   (Journal Full-text)

Spike output in many neuronal cell types is affected by low-voltage-activated T-type calcium currents arising from the Ca(v)3.1, Ca(v)3.2 and Ca(v)3.3 channel subtypes and their splice isoforms. The contributions of T-type current to cell output is often proposed to reflect a differential distribution of channels to somatic and dendritic compartments, but the subcellular distribution of the various rat T-type channel isoforms has not been fully determined. We used subtype-specific Ca(v)3 polyclonal antibodies to determine their distribution in key regions of adult Sprague-Dawley rat brain thought to exhibit T-type channel expression, and in particular, dendritic low-voltage-activated responses. We found a selective subcellular distribution of Ca(v)3 channel proteins in cell types of the neocortex and hippocampus, thalamus, and cerebellar input and output neurons. In general, the Ca(v)3.1 T-type channel immunolabel is prominent in the soma/proximal dendritic region and Ca(v)3.2 immunolabel in the soma and proximal-mid dendrites. Ca(v)3.3 channels are distinct in distributing to the soma and over extended lengths of the dendritic arbor of particular cell types. Ca(v)3 distribution overlaps with cell types previously established to exhibit rebound burst discharge as well as those not recognized for this activity. Additional immunolabel in the region of the nucleus in particular cell types was verified as corresponding to Ca(v)3 antigen through analysis of isolated protein fractions. These results provide evidence that different Ca(v)3 channel isoforms may contribute to low-voltage-activated calcium-dependent responses at the somatic and dendritic level, and the potential for T-type calcium channels to contribute to multiple aspects of neuronal activity.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dendrite  IDA 2308880 RGD 
neuronal cell body  IDA 2308880 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cacna1g  (calcium voltage-gated channel subunit alpha1 G)


Additional Information