RGD Reference Report - A multifunctional transcription factor (A1p145) regulates the smooth muscle phenotype in mesangial cells. - Rat Genome Database

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A multifunctional transcription factor (A1p145) regulates the smooth muscle phenotype in mesangial cells.

Authors: Takeoka, H  Iehara, N  Uematsu-Yanagita, M  Abe, H  Sunamoto, M  Yamada, Y  Kita, T  Doi, T 
Citation: Takeoka H, etal., Biochem Biophys Res Commun. 1998 Nov 18;252(2):290-5.
RGD ID: 2307113
Pubmed: PMID:9826522   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1998.9530   (Journal Full-text)

A1p145, a novel DNA binding protein for type IV collagen gene (COL4), has multiple functions including DNA replication factor C and DNA binding for several other genes. To elucidate the mechanisms underlying the differentiation process of mesangial cells (MCs), we investigated the effects of A1p145 on rat MCs. Cells in the early passages showed a smooth muscle-like phenotype such as low cell turnover, high levels of expression for COL4, and smooth muscle alpha-actin (SMA). Cells in the late passages lost their phenotype. The amount of binding activity to COL4 promoter was inversely correlated with the level of COL4 mRNA. Introduction of antisense for A1p145 into late passage cells enhanced the levels of mRNA for COL4 and SMA. The levels of proliferating cell nuclear antigen mRNA were also suppressed. These results suggest that A1p145 is a negative transcription factor for COL4 and may be a phenotypic modulator.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of DNA-templated transcription  IMP 2307113 RGD 
positive regulation of DNA-templated transcription  IMP 2307113 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
double-stranded DNA binding  IDA 2307113 RGD 
sequence-specific DNA binding  IDA 2307113 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rfc1  (replication factor C subunit 1)


Additional Information