RGD Reference Report - Chronic ethanol consumption resulting in the downregulation of insulin receptor-beta subunit, insulin receptor substrate-1, and glucose transporter 4 expression in rat cardiac muscles. - Rat Genome Database

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Chronic ethanol consumption resulting in the downregulation of insulin receptor-beta subunit, insulin receptor substrate-1, and glucose transporter 4 expression in rat cardiac muscles.

Authors: Limin, T  Hou, X  Liu, J  Zhang, X  Sun, N  Gao, L  Zhao, J 
Citation: Limin T, etal., Alcohol. 2009 Feb;43(1):51-8.
RGD ID: 2307017
Pubmed: PMID:19185210   (View Abstract at PubMed)
DOI: DOI:10.1016/j.alcohol.2008.11.001   (Journal Full-text)

The objective of this study was to investigate the effect of chronic ethanol intake on the expression of insulin receptor beta subunit (IR-beta), insulin receptor substrate-1 (IRS-1), and glucose transporter 4 (Glut4) in rat cardiac muscle. Forty-eight male Wistar rats were randomly classified into four groups and to each group, ethanol was administered daily at the respective doses of 0 (control, C), 0.5 g kg(-1) (low ethanol, L), 2.5 g kg(-1) (middle ethanol, M), and 5 g kg(-1) (high ethanol, H). Twenty-two weeks later, the rats were anesthetized, and the left ventricle muscles were excised. The IR-beta, IRS-1, and Glut4 mRNA levels in the cardiac muscle tissues were detected by reverse-transcription polymerase chain reaction (RT-PCR); the IR-beta, tyrosine phosphorylation of IR-beta (PY-IR-beta), IRS-1, tyrosine phosphorylation of IRS-1 (PY-IRS-1), and Glut4 protein levels were measured by Western blotting. Compared to the control group, the IR-beta, IRS-1, and Glut4 mRNA levels in groups H and M decreased remarkably. In addition, the protein levels of IR-beta, IRS-1, and Glut4 showed a corresponding decline in ethanol-treated groups, especially in group H. Moreover, the PY-IR-beta and PY-IRS-1 protein levels decreased in the hearts of ethanol-treated rats with corresponding changes in the IR-beta and IRS-1 protein levels. The present study showed that chronic ethanol intake could downregulate the expression levels of IR-beta, IRS-1, and Glut4 in rat cardiac muscles.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to ethanol  IEP 2307017IR-beta in cardiac muscleRGD 

Objects Annotated

Genes (Rattus norvegicus)
Insr  (insulin receptor)


Additional Information