RGD Reference Report - Purification, identification and phosphorylation of annexin I from rat liver mitochondria. - Rat Genome Database

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Purification, identification and phosphorylation of annexin I from rat liver mitochondria.

Authors: Yoshii, K  Sugimoto, K  Tai, Y  Konishi, R  Tokuda, M 
Citation: Yoshii K, etal., Acta Med Okayama. 2000 Apr;54(2):57-65.
RGD ID: 2306913
Pubmed: PMID:10806526   (View Abstract at PubMed)

Annexin was purified from rat liver mitochondria to an apparent homogeneity with a molecular weight of 35 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified mitochondrial annexin (AXmito) was identified as annexin I by an immunoblot analysis using anti-annexin I antibody. The inhibitory effect of AXmito I on porcine pancreatic phospholipase A2 activity was as potent as that of bovine lung annexin I. The presence of annexin I in mitochondria was confirmed by an electron-microscopic study. AXmito I was shown to be phosphorylated by intrinsic protein tyrosine kinases on its tyrosine residues. This annexin was also phosphorylated by protein kinase C.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitochondrial membrane  IDA 2306913 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
phospholipase A2 inhibitor activity  IDA 2306913 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Anxa1  (annexin A1)


Additional Information