RGD Reference Report - Macrophage-inflammatory protein-1alpha regulates preosteoclast differentiation in vitro. - Rat Genome Database

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Macrophage-inflammatory protein-1alpha regulates preosteoclast differentiation in vitro.

Authors: Scheven, BA  Milne, JS  Hunter, I  Robins, SP 
Citation: Scheven BA, etal., Biochem Biophys Res Commun. 1999 Jan 27;254(3):773-8.
RGD ID: 2303108
Pubmed: PMID:9920817   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1998.9909   (Journal Full-text)

A validated in vitro system was used to investigate the nature of osteoclast-inducing growth factors (OGF) present in fetal rat calvarial conditioned medium (RCCM). Evidence is presented here that macrophage inflammatory protein-1alpha (MIP-1alpha), a member of the C-C chemokine family, is an essential factor for the induction of osteoclast differentiation in this system. Specific polyclonal antibodies against MIP-1alpha significantly inhibited development of TRAP-positive osteoclast precursors and multinucleated osteoclasts induced by RCCM. Anti-MIP-1alpha antibody treatment was accompanied by an increase in the number of macrophage-like cells, suggesting that bone-derived MIP-1alpha is involved in the direction of preosteoclast formation with an inhibitory action on progenitor cell proliferation. Reverse-phase HPLC of RCCM resolved multiple fractions with OGF activity. OGF fractions separated at low acetonitrile (AcN) concentrations (

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of osteoclast differentiation  IDA 2303108; 2303108 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccl3  (C-C motif chemokine ligand 3)
Ccl5  (C-C motif chemokine ligand 5)


Additional Information