RGD Reference Report - Isotope-exchange evidence that glucose 6-phosphate inhibits rat-muscle hexokinase II at an allosteric site. - Rat Genome Database

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Isotope-exchange evidence that glucose 6-phosphate inhibits rat-muscle hexokinase II at an allosteric site.

Authors: Gregoriou, M  Trayer, IP  Cornish-Bowden, A 
Citation: Gregoriou M, etal., Eur J Biochem. 1983 Aug 1;134(2):283-8.
RGD ID: 2302671
Pubmed: PMID:6603359   (View Abstract at PubMed)

The flux ratio for hexokinase type II from rat muscle, i.e. the rate of conversion of glucose 6-phosphate molecules into ATP molecules divided by the simultaneous rate of conversion of glucose 6-phosphate molecules into glucose molecules, increases with the MgATP concentration but is independent of the glucose concentration. This behaviour requires that glucose must bind before MgATP when the reaction is proceeding in the normal physiological direction, i.e. phosphorylation of glucose. Although at low non-inhibitory glucose 6-phosphate concentrations the flux ratio increases linearly with the MgATP concentration, the dependence becomes non-linear, with a slope that increases with the MgATP concentration, at glucose 6-phosphate concentrations above 1 mM. This behaviour does not permit glucose 6-phosphate to act only as a normal product inhibitor. Instead, it seems to require glucose 6-phosphate to act as an allosteric inhibitor and for a second site for binding of MgATP to exist. Measurements of the flux from ATP to glucose 6-phosphate and to ADP showed no dependence of the flux ratio on the concentrations of either glucose 6-phosphate or ADP. This result does not permit the order of product-release steps in this direction to be determined, but shows that the second product is released virtually instantaneously after first.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
carbohydrate phosphorylation  IDA 2302671 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ATP binding  IDA 2302671 RGD 
glucose binding  IDA 2302671 RGD 
hexokinase activity  IDA 2302671 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hk2  (hexokinase 2)


Additional Information