RGD Reference Report - FMS-like tyrosine kinase 3 interacts with the glucocorticoid receptor complex and affects glucocorticoid dependent signaling. - Rat Genome Database

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FMS-like tyrosine kinase 3 interacts with the glucocorticoid receptor complex and affects glucocorticoid dependent signaling.

Authors: Asadi, A  Hedman, E  Widen, C  Zilliacus, J  Gustafsson, JA  Wikstrom, AC 
Citation: Asadi A, etal., Biochem Biophys Res Commun. 2008 Apr 11;368(3):569-74. Epub 2008 Feb 7.
RGD ID: 2302206
Pubmed: PMID:18261979   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2008.01.146   (Journal Full-text)

The glucocorticoid receptor (GR) forms part of a multiprotein complex consisting of chaperones and proteins active in glucocorticoid signaling and other pathways. By immunoaffinity purification of GR, followed by Edman sequencing and Western blotting, we identified the FMS-like tyrosine kinase 3 (Flt3) as a GR-interacting protein in rat liver and hepatoma cells. Flt3 interacts with both non-liganded and liganded GR. The DNA-binding domain of GR is sufficient for Flt3 interaction as shown by GST-pull down experiments. Studies of the effects of Flt3 and its ligand FL in glucocorticoid-driven reporter-gene assays in Cos7 cells, show that co-transfection with Flt3 and FL potentiates glucocorticoid effects. Treatment with FL had no effect on GR location and Dex induced translocation of GR was unaffected by FL. In summary, GR and Flt3 interact, affecting GR signaling. This novel cross-talk between GR and a hematopoietic growth factor might also imply glucocorticoid effects on Flt3-mediated signaling.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to glucocorticoid stimulus  IEP 2302206 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex  IDA 2302206 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Flt3  (Fms related receptor tyrosine kinase 3)
Nr3c1  (nuclear receptor subfamily 3, group C, member 1)


Additional Information