RGD Reference Report - Integrin-linked kinase induces both senescence-associated alterations and extracellular fibronectin assembly in aging cardiac fibroblasts. - Rat Genome Database

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Integrin-linked kinase induces both senescence-associated alterations and extracellular fibronectin assembly in aging cardiac fibroblasts.

Authors: Chen, X  Li, Z  Feng, Z  Wang, J  Ouyang, C  Liu, W  Fu, B  Cai, G  Wu, C  Wei, R  Wu, D  Hong, Q 
Citation: Chen X, etal., J Gerontol A Biol Sci Med Sci. 2006 Dec;61(12):1232-45.
RGD ID: 2301768
Pubmed: PMID:17234816   (View Abstract at PubMed)

Integrin-linked kinase (ILK) is an integrin-binding cytoplasmic protein that is involved in regulating numerous cellular processes and extracellular matrix accumulation. We reported that ILK may be involved in cellular senescence, but whether ILK is the cause of senescence or an accompanying phenomenon still remains to be explored. Here, RNA interference and gene transfer techniques were used to knock down and overexpress ILK in 3-month-old and 28-month-old rat primary cardiac fibroblasts. The results show that, in younger cells, ILK overexpression induces larger cell shapes, lower proliferation capacity, and higher levels of enzymatic beta-galactosidase activity, and increases basal p53 and p21 protein levels, whereas knock-down of ILK prevents phenotypic changes typical of senescence in aging cells. In addition, ILK could induce the cytoskeleton proteins to organize into dense, thick bundles of filaments, which contribute to cellular enlargement and extracellular fibronectin assembly. The results indicate that ILK can accelerate the process of cellular senescence.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cellular senescence  IMP 2301768 RGD 
regulation of actin cytoskeleton organization  IMP 2301768positive regulation of alpha smooth muscle actin organization in aging cellsRGD 
supramolecular fiber organization  IMP 2301768promotes the extracellular assembly of fibronectin in aging cellsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ilk  (integrin-linked kinase)


Additional Information