RGD Reference Report - The distribution of glutamine synthetase in subcellular fractions of rat brain. - Rat Genome Database

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The distribution of glutamine synthetase in subcellular fractions of rat brain.

Authors: Dennis, SC  Lai, JC  Clark, JB 
Citation: Dennis SC, etal., Brain Res. 1980 Sep 22;197(2):469-75.
RGD ID: 2301549
Pubmed: PMID:6105901   (View Abstract at PubMed)

The subcellular localization of glutamine synthetase, an enzyme fundamental to the compartmentation of glutamate hypothesis, was investigated using brain tissue of adult rats. The distribution of this enzyme in relation to the distribution of glucose-6-phosphatase, glutamate dehydrogenase and acetycholine esterase was studied using a fractionation scheme which had been previously extensively characterized in terms of intramitochondrial enzyme complements. Glutamine synthetase was found to be predominantly localized at the nerve terminal and a number of results suggested a possibble association with the synaptic membrane. The observations are discussed in relation to the compartmentation of glutamate metabolism. Acetate and ammonia are precursors of the 'small' pool of glutamate from which most of the synthesis of glutamine occurs. Since one population of synaptic mitochondria has previously been shown to be enriched in glutamate dehydrogenase and acetyl CoA synthetase and in view of the current observtions that synaptosomes are probably in association with a large proportion of brain glutamine synthetase, it is tentatively suggested that the synaptic complex represents at least in part the site of the 'small' glutamate pool.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axon terminus  IDA 2301549 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Glul  (glutamate-ammonia ligase)


Additional Information