RGD Reference Report - Real-time PCR quantitation of FE65 a beta-amyloid precursor protein-binding protein after traumatic brain injury in rats. - Rat Genome Database

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Real-time PCR quantitation of FE65 a beta-amyloid precursor protein-binding protein after traumatic brain injury in rats.

Authors: Iino, M  Nakatome, M  Ogura, Y  Fujimura, H  Kuroki, H  Inoue, H  Ino, Y  Fujii, T  Terao, T  Matoba, R 
Citation: Iino M, etal., Int J Legal Med. 2003 Jun;117(3):153-9. Epub 2003 Apr 18.
RGD ID: 2301208
Pubmed: PMID:12707777   (View Abstract at PubMed)
DOI: DOI:10.1007/s00414-003-0370-y   (Journal Full-text)

In cases of traumatic brain injury (TBI) in which the patient survived for only a short period of time and was without macroscopic changes at autopsy, it is difficult to diagnose TBI. To detect early diagnostic markers of diffuse axonal injury (DAI), real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in an experimental head trauma model of rat was chosen. The beta-amyloid precursor protein (beta-APP) is a well-known diagnostic marker of DAI which can be detected by immunolabeling as early as 1.5 h after injury. beta-APP has a binding protein, FE65, which is expressed in the brain of Alzheimer's disease patients along with beta-APP, but no involvement with brain injury has been reported. Neuron-specific enolase (NSE) is also a useful marker of DAI. We found that FE65 expression increased dramatically as early as 30 min after injury and decreased after peaking 1 h post-injury, although NSE showed no significant changes. These results suggest that real-time PCR of FE65 mRNA is useful for the diagnosis of DAI in forensic cases.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Brain Injuries  ISOApbb1 (Rattus norvegicus)2301208; 2301208mRNA:increased expression:brainRGD 
Brain Injuries  IEP 2301208mRNA:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Apbb1  (amyloid beta precursor protein binding family B member 1)

Genes (Mus musculus)
Apbb1  (amyloid beta precursor protein binding family B member 1)

Genes (Homo sapiens)
APBB1  (amyloid beta precursor protein binding family B member 1)


Additional Information