RGD Reference Report - Effect of early insulin therapy on nuclear factor kappaB and cytokine gene expressions in the liver and skeletal muscle of high-fat diet, streptozotocin-treated diabetic rats.

General

Effect of early insulin therapy on nuclear factor kappaB and cytokine gene expressions in the liver and skeletal muscle of high-fat diet, streptozotocin-treated diabetic rats.
Authors:	Bi, Y Sun, WP Chen, X Li, M Liang, H Cai, MY Zhu, YH He, XY Xu, F Weng, JP
Citation:	Bi Y, etal., Acta Diabetol. 2008 May 24.
RGD ID:	2298780
Pubmed:	PMID:18500427 (View Abstract at PubMed)
DOI:	DOI:10.1007/s00592-008-0038-7 (Journal Full-text)
To clarify the effect of early insulin therapy on nuclear factor kappaB (NFkappaB) pathway and inflammatory cytokine responses in the liver and skeletal muscle in type 2 diabetes. High-fat diet and low dose streptozotocin (STZ) induced diabetic rats were given NPH insulin or gliclazide for 3 weeks initiated at the 3rd day after STZ injection as early treatment and NPH for 3 weeks at 1 month as late treatment. Intraperitoneal glucose tolerance test (IPGTT) was performed at 3rd day after the end of treatment. Early interventions caused a decrease in glucose-insulin index in IPGTT, promoted glucose transporter 4 (Glut4) gene and protein expressions in muscle and reduced phosphoenolpyruvate carboxykinase (PEPCK) protein levels in the liver. There was an increase in inhibitor kappaB (IkappaBalpha) protein and a decrease in NFkappaB p65 DNA binding activity. A decreased level in mRNAs encoding tumor necrosis factor (TNF)alpha in the liver and muscle and interleukin (IL)-1beta in the liver were observed. Our results suggested that early insulin treatment inhibits NFkappaB activity and inflammatory cytokine responses in the liver and skeletal muscle that were involved in the amelioration of insulin resistance in type 2 diabetic rats.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
Experimental Diabetes Mellitus		ISO	Rela (Rattus norvegicus)	2298780; 2298780	protein:increased expression:nucleus more ...	RGD
Experimental Diabetes Mellitus		IEP		2298780	protein:increased expression:nucleus more ...	RGD

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Biological Process

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
response to insulin		IEP		2298780		RGD

Objects Annotated

Genes (Rattus norvegicus)

Rela (RELA proto-oncogene, NF-kB subunit)

Genes (Mus musculus)

Rela (v-rel reticuloendotheliosis viral oncogene homolog A (avian))

Genes (Homo sapiens)

RELA (RELA proto-oncogene, NF-kB subunit)

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Effect of early insulin therapy on nuclear factor kappaB and cytokine gene expressions in the liver and skeletal muscle of high-fat diet, streptozotocin-treated diabetic rats.