RGD Reference Report - BAG-1 expression in normal and neoplastic endometrium.

General

BAG-1 expression in normal and neoplastic endometrium.
Authors:	Moriyama, T Littell, RD Debernardo, R Oliva, E Lynch, MP Rueda, BR Duska, LR
Citation:	Moriyama T, etal., Gynecol Oncol. 2004 Aug;94(2):289-95.
RGD ID:	2293886
Pubmed:	PMID:15297164 (View Abstract at PubMed)
DOI:	DOI:10.1016/j.ygyno.2004.04.026 (Journal Full-text)
OBJECTIVE: BAG-1 has anti-apoptotic actions and is known to bind BCL-2 and steroid receptors. High levels of BAG-1 have been implicated as a prognostic indicator in breast cancer. Whether this observation can be generalized to endometrial cancer remains unknown. METHODS: IRB permission was obtained for use of human discarded tissue. Immunohistochemical analyses were performed on: proliferative endometrium (PEM, 6), secretory endometrium (SEM, 28), "low-grade" neoplastic lesions (complex atypical hyperplasia and grade 1 endometrial adenocarcinomas) (19), and "high-grade" cancers (grade 2 and 3 endometrial adenocarcinomas) (13). The level of total BAG-1 and its isoforms was evaluated by Western blot in lysates from Ishikawa cells (grade 1), MFE 296 cells (grade 2), and SK-UT(2) cells (grade 3). RESULTS: The proportion of "high-grade" cancers with positive cytoplasmic staining for BAG-1 was higher than that of secretory endometrium (P = 0.006). Additionally, the proportion of specimens with positive staining for nuclear BAG-1 expression was significantly higher among high-grade carcinoma specimens compared to secretory specimens (P = 0.009). A high proportion (91%) of all specimens were positive for BCL-2, limiting the ability to subcategorize the other variables analyzed. There was no relationship between positive nuclear BAG-1 expression and either estrogen receptor (ER) or progesterone receptor (PR) expression. BAG-1 was expressed in the three cell lines evaluated and total BAG-1 level was not different among the different cell lines. CONCLUSION: BAG-1 is expressed in the endometrium. High-grade cancers stain more frequently than secretory endometrium for both cytoplasmic and nuclear BAG-1 expression, perhaps indicating an association between expression of BAG-1 and prognosis.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
endometrial cancer		IEP		2293886	protein:increased expression:endometrium	RGD
endometrial cancer		ISO	BAG1 (Homo sapiens)	2293886; 2293886	protein:increased expression:endometrium	RGD

Objects Annotated

Genes (Rattus norvegicus)

Bag1 (BAG cochaperone 1)

Genes (Mus musculus)

Bag1 (BCL2-associated athanogene 1)

Genes (Homo sapiens)

BAG1 (BAG cochaperone 1)

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BAG-1 expression in normal and neoplastic endometrium.