RGD Reference Report - Association of MEK1 with p21ras.GMPPNP is dependent on B-Raf. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Association of MEK1 with p21ras.GMPPNP is dependent on B-Raf.

Authors: Moodie, SA  Paris, MJ  Kolch, W  Wolfman, A 
Citation: Moodie SA, etal., Mol Cell Biol. 1994 Nov;14(11):7153-62.
RGD ID: 2293305
Pubmed: PMID:7935430   (View Abstract at PubMed)
PMCID: PMC359249   (View Article at PubMed Central)

We have previously reported that immobilized p21ras forms a GMPPNP-dependent complex with a MEK activity. Furthermore, the association of the MEK activity was found to be independent of the presence of Raf-1. We have extended those observations to show that MEK1 is the MEK activity previously described to associate with immobilized p21ras.GMPPNP. The association between MEK1 and immobilized p21ras.GMPPNP increased its specific activity towards p42MAPK. We detected the specific association of B-Raf with immobilized p21ras.GMPPNP. In contrast to Raf-1-immunodepleted lysates, preclearance of the cytosolic B-Raf significantly reduced, by 96%, the amount of MEK1 activity associated with immobilized p21ras.GMPPNP. The decrease in MEK1 activity correlated with complete loss in the binding of both B-Raf and MEK1 proteins with immobilized p21ras.GMPPNP. These data suggest that the p21ras.GMPPNP-dependent activation of MEK1 in brain extracts is dependent on the presence of the B-Raf protein kinase.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of MAPK cascade  IDA 2293305 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
MAP kinase kinase activity  IDA 2293305 RGD 
small GTPase binding  IPIHRAS (Homo sapiens)2293305 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Map2k1  (mitogen activated protein kinase kinase 1)


Additional Information