RGD Reference Report - Signaling mechanisms of daidzein-induced axonal outgrowth in hippocampal neurons. - Rat Genome Database

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Signaling mechanisms of daidzein-induced axonal outgrowth in hippocampal neurons.

Authors: Wang, P  Jeng, CJ  Chien, CL  Wang, SM 
Citation: Wang P, etal., Biochem Biophys Res Commun. 2008 Feb 8;366(2):393-400. Epub 2007 Dec 4.
RGD ID: 2293300
Pubmed: PMID:18062921   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2007.11.147   (Journal Full-text)

We aim to study the mechanisms underlying the neurotrophic effect of daidzein (Dz) in hippocampal neurons. Dz-enhanced axonal outgrowths manifested growth cone formation and increased immunostaining intensity of growth-associated protein 43 (GAP-43) in growth cones. Consistent with this, Dz increased GAP-43 phosphorylation and its membrane translocation without affecting total GAP-43 levels. In the presence of Dz, significant increase in the immunoreactivity for estrogen receptor (ER) beta, but not ERalpha, was observed on the membrane of cell bodies and growing axons. Dz also induced the activation of protein kinase C alpha (PKCalpha), which was inhibited by the ICI182,780 pretreatment. Similarly, Dz-promoted axonal elongation was blocked by ICI182,780 and Go6976. Moreover, Dz-stimulated activation of GAP-43 was specifically abolished by Go6976, suggesting PKCalpha being the upstream effector of GAP-43. Taken together, our data suggest that Dz triggers an ERbeta/PKCalpha/GAP-43 signaling cascade to promote axonal outgrowths in cultured hippocampal neurons.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
central nervous system neuron axonogenesis  IMP 2293300 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prkca  (protein kinase C, alpha)


Additional Information