RGD Reference Report - Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis. - Rat Genome Database

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Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis.

Authors: Acharya, U  Mallabiabarrena, A  Acharya, JK  Malhotra, V 
Citation: Acharya U, etal., Cell. 1998 Jan 23;92(2):183-92.
RGD ID: 2293212
Pubmed: PMID:9458043   (View Abstract at PubMed)

We have developed an assay using permeabilized cells to monitor fragmentation of the Golgi complex that occurs during mitosis. Golgi stacks, in permeabilized interphase normal rat kidney (NRK) cells, upon incubation with mitotic extracts undergo extensive fragmentation, and the fragmented Golgi membranes are dispersed throughout the cytoplasm. We find that the continued presence of p34cdc2, the mitosis initiation kinase, is not necessary for Golgi fragmentation. Instead, fragmentation depends on cytosolic mitogen-activated protein kinase kinase 1 (MEK1 or MAPKK1). However, the known cytoplasmic substrates for MEK1, ERK1, and ERK2 are not required for this process. Interestingly, we find a Golgi-associated ERK, which we propose as the likely target for MEK1 in Golgi fragmentation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Golgi inheritance  IMP 2293212Golgi fragmentation during mitosisRGD 

Objects Annotated

Genes (Rattus norvegicus)
Map2k1  (mitogen activated protein kinase kinase 1)


Additional Information