RGD Reference Report - Lidocaine increases phosphorylation of focal adhesion kinase in rat hippocampal slices. - Rat Genome Database

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Lidocaine increases phosphorylation of focal adhesion kinase in rat hippocampal slices.

Authors: Dahmani, S  Reynaud, C  Tesniere, A  Rouelle, D  Desmonts, JM  Mantz, J 
Citation: Dahmani S, etal., Eur J Pharmacol. 2004 Apr 5;489(1-2):55-8.
RGD ID: 2292608
Pubmed: PMID:15063155   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ejphar.2004.02.012   (Journal Full-text)

We examined the effect of lidocaine on phosphorylation of the tyrosine kinase focal adhesion kinase (PP125FAK) in rat hippocampal slices by immunoblotting with both antiphosphotyrosine and specific anti-PP125FAK antibodies in the presence of tetrodotoxin (1 microM). Lidocaine induced a concentration-related increase in tyrosine phosphorylation of the 125-kDa band corresponding to PP125FAK phosphorylation (EC50 value=0.39+/-0.09 microM, maximal effect=169+/-28% of control, P<0.001). This effect was sensitive to neither the N-methyl-D-aspartate (NMDA) receptor antagonist dizocilpine (MK 801, 10 microM) nor the inhibitor of the ryanodine receptor dantrolene (30 microM). In contrast, it was completely blocked by the protein kinase C (PKC) inhibitors chelerythrin, bisindolylmaleimide I (GF 109203X) and bisindolylmaleimide IX (RO-318220, 10 microM). We conclude that lidocaine increases phosphorylation of the tyrosine kinase PP125FAK in the rat hippocampus by a tetrotoxin (TTX)-insensitive mechanism which involves activation of PKC.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to organic cyclic compound  IEP 2292608 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptk2  (protein tyrosine kinase 2)


Additional Information