RGD Reference Report - Time-related changes in constitutive and inducible nitric oxide synthases in the rat striatum in a model of Huntington's disease. - Rat Genome Database

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Time-related changes in constitutive and inducible nitric oxide synthases in the rat striatum in a model of Huntington's disease.

Authors: Aguilera, P  Chanez-Cardenas, ME  Floriano-Sanchez, E  Barrera, D  Santamaria, A  Sanchez-Gonzalez, DJ  Perez-Severiano, F  Pedraza-Chaverri, J  Jimenez, PD 
Citation: Aguilera P, etal., Neurotoxicology. 2007 Nov;28(6):1200-7. Epub 2007 Aug 6.
RGD ID: 2292130
Pubmed: PMID:17850874   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuro.2007.07.010   (Journal Full-text)

Excitotoxicity and oxidative stress are mechanisms involved in the neuronal cell death induced by the intrastriatal injection of quinolinic acid (QUIN) as a model of Huntington's disease. Production of nitric oxide by nitric oxide synthase (NOS) has been proposed to participate in QUIN-induced neurotoxicity; however, the precise role of NOS in QUIN-induced toxicity still remains controversial. In order to provide further information on the role of NOS isoforms in QUIN toxicity, we performed real time RT-PCR and immunohistochemistry of inducible NOS (iNOS), endothelial NOS (eNOS) and neuronal NOS (nNOS) and determined Ca(2+)-dependent and Ca(2+)-independent NOS activity in a temporal course (3-48h), after an intrastriatal injection of QUIN to rats. NOS isoforms exhibited a transitory expression of mRNA and protein after QUIN infusion: eNOS increased between 3 and 24h, iNOS between 12 and 24h, while nNOS at 35 and 48h. Ca(2+)-independent activity (iNOS) did not show any change, while Ca(2+)-dependent activity (constitutive NOS: eNOS/nNOS) exhibited increased levels at 3h. Our results support the participation of Ca(2+)-dependent NOS isoforms during the toxic events produced at early times after QUIN injection.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Huntington's disease  ISONos3 (Rattus norvegicus)2292130; 2292130mRNA:increased expression:striatum (rat)RGD 
Huntington's disease  IEP 2292130mRNA:increased expression:striatum (rat)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to organic cyclic compound  IEP 2292130 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nos3  (nitric oxide synthase 3)

Genes (Mus musculus)
Nos3  (nitric oxide synthase 3, endothelial cell)

Genes (Homo sapiens)
NOS3  (nitric oxide synthase 3)


Additional Information