RGD Reference Report - FasL, Fas, and death-inducing signaling complex (DISC) proteins are recruited to membrane rafts after spinal cord injury. - Rat Genome Database

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FasL, Fas, and death-inducing signaling complex (DISC) proteins are recruited to membrane rafts after spinal cord injury.

Authors: Davis, AR  Lotocki, G  Marcillo, AE  Dietrich, WD  Keane, RW 
Citation: Davis AR, etal., J Neurotrauma. 2007 May;24(5):823-34.
RGD ID: 2290177
Pubmed: PMID:17518537   (View Abstract at PubMed)
DOI: DOI:10.1089/neu.2006.0227   (Journal Full-text)

The Fas/CD95 receptor-ligand system plays an essential role in apoptosis that contributes to secondary damage after spinal cord injury (SCI), but the mechanism regulating the efficiency of FasL/Fas signaling in the central nervous system (CNS) is unknown. Here, FasL/Fas signaling complexes in membrane rafts were investigated in the spinal cord of adult female Fischer rats subjected to moderate cervical SCI and sham operation controls. In sham-operated animals, a portion of FasL, but not Fas was present in membrane rafts. SCI resulted in FasL and Fas translocation into membrane raft microdomains where Fas associates with the adaptor proteins Fas-associated death domain (FADD), caspase-8, cellular FLIP long form (cFLIPL ), and caspase-3, forming a death-inducing signaling complex (DISC). Moreover, SCI induced expression of Fas in clusters around the nucleus in both neurons and astrocytes. The formation of the DISC signaling platform leads to rapid activation of initiator caspase-8 and effector caspase-3, and the modification of signaling intermediates such as FADD and cFLIP(L) . Thus, FasL/Fas-mediated signaling after SCI is similar to Fas expressing Type I cell apoptosis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Spinal Cord Injuries  ISOFas (Rattus norvegicus)2290177; 2290177protein:increased expression:cervical spinal cordRGD 
Spinal Cord Injuries  IEP 2290177protein:increased expression:cervical spinal cordRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
death-inducing signaling complex  IDA 2290177; 2290177; 2290177; 2290177; 2290177 RGD 
perinuclear region of cytoplasm  IDA 2290177 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Casp3  (caspase 3)
Casp8  (caspase 8)
Cflar  (CASP8 and FADD-like apoptosis regulator)
Fadd  (Fas associated via death domain)
Fas  (Fas cell surface death receptor)

Genes (Mus musculus)
Fas  (Fas cell surface death receptor)

Genes (Homo sapiens)
FAS  (Fas cell surface death receptor)

Objects referenced in this article
Gene Faslg Fas ligand Rattus norvegicus

Additional Information