RGD Reference Report - Measurement of catechol-O-methyltransferase activity in the brain of Dahl salt-sensitive rats. - Rat Genome Database

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Measurement of catechol-O-methyltransferase activity in the brain of Dahl salt-sensitive rats.

Authors: Hirano, Y  Tsunoda, M  Shimosawa, T  Fujita, T  Funatsu, T 
Citation: Hirano Y, etal., Biol Pharm Bull. 2007 Nov;30(11):2178-80.
RGD ID: 2289729
Pubmed: PMID:17978496   (View Abstract at PubMed)

Metabolism by catechol-O-methyltransferase (COMT) is one of the inactivation pathways of catecholamines (CAs), which are important hormones in regulating blood pressure both in central and in peripheral sympathetic nerve endings. We have reported the rapid determination method of COMT activity using high-performance liquid chromatography (HPLC)-fluorescence detection. In the present study, we applied the method to brain tissues, cerebral cortex, cerebellum, hypophysis and hypothalamus. COMT activities were assessed by measuring normetanephrine with the use of norepinephrine as an endogenous substrate. We examined the COMT activities in the brains of Dahl salt-sensitive (DS) rats given normal-salt or high-salt diet for 13 weeks, and found that membrane-bound COMT activities in the cerebral cortex were significantly reduced in high-salt loaded DS rats compared with normal-salt loaded DS rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hypertension  ISOComt (Rattus norvegicus)2289729; 2289729protein:increased expression:cerebral cortexRGD 
hypertension  IEP 2289729protein:increased expression:cerebral cortexRGD 

Objects Annotated

Genes (Rattus norvegicus)
Comt  (catechol-O-methyltransferase)

Genes (Mus musculus)
Comt  (catechol-O-methyltransferase)

Genes (Homo sapiens)
COMT  (catechol-O-methyltransferase)


Additional Information