RGD Reference Report - Reduced metastasis-suppressor gene mRNA-expression in breast cancer brain metastases. - Rat Genome Database

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Reduced metastasis-suppressor gene mRNA-expression in breast cancer brain metastases.

Authors: Stark, AM  Tongers, K  Maass, N  Mehdorn, HM  Held-Feindt, J 
Citation: Stark AM, etal., J Cancer Res Clin Oncol. 2005 Mar;131(3):191-8. Epub 2004 Dec 8.
RGD ID: 2289400
Pubmed: PMID:15592684   (View Abstract at PubMed)
DOI: DOI:10.1007/s00432-004-0629-9   (Journal Full-text)

PURPOSE: Brain metastases are an increasingly common complication in breast cancer patients. The Metastasis Suppressor Genes (MSG) Nm23, KISS1, KAI1, BRMS1, and Mkk4 have been associated with the metastatic potential of breast cancer in vitro and in vivo. METHODS: The mRNA expression of Nm23, KISS1, KAI1, BRMS1, and Mkk4 in fresh frozen tissue samples of brain metastases from ductal invasive breast cancer specimens was examined in relation to primary tumors. In a first step, mRNA expression screening was carried out using a semi-quantitative RT-PCR approach, in a second step quantitative real-time RT-PCR was performed on selected specimens. By immunohistochemical staining, gene products were visualized on the protein level. RESULTS: Semi-quantitative RT-PCR revealed reduced mRNA expression of Nm23, KISS1, KAI1, BRMS, and Mkk4 in brain metastases. Results for KISS1, KAI1, BRMS, and Mkk4 were confirmed by real-time RT-PCR. In detail, mRNA expression reduction in breast cancer brain metastases was tenfold. Expression of MSG could be confirmed by immunohistochemical staining on protein level. CONCLUSIONS: Our investigations revealed significantly reduced mRNA expression of metastases suppressor genes KISS1, KAI1, BRMS1, and Mkk4 in breast cancer brain metastasis. Particularly, in the case of KISS1 and Mkk4, an important role for future treatment of patients with breast cancer brain metastatic lesions can be assumed.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
invasive ductal carcinoma disease_progressionIEP 2289400; 2289400; 2289400mRNA and protein:decreased expression:brain metastases:10-fold lower expression in metastases vs primary tumorsRGD 
invasive ductal carcinoma disease_progressionISOBRMS1 (Homo sapiens)2289400; 2289400 RGD 
invasive ductal carcinoma disease_progressionISOKISS1 (Homo sapiens)2289400; 2289400 RGD 
invasive ductal carcinoma disease_progressionISOMAP2K4 (Homo sapiens)2289400; 2289400 RGD 
Neoplasm Metastasis  IEP 2289400associated with Carcinoma more ...RGD 
Neoplasm Metastasis  ISOCD82 (Homo sapiens)2289400; 2289400associated with Carcinoma more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Brms1  (BRMS1, transcriptional repressor and anoikis regulator)
Cd82  (Cd82 molecule)
Kiss1  (KiSS-1 metastasis-suppressor)
Map2k4  (mitogen activated protein kinase kinase 4)

Genes (Mus musculus)
Brms1  (breast cancer metastasis-suppressor 1)
Cd82  (CD82 antigen)
Kiss1  (KiSS-1 metastasis-suppressor)
Map2k4  (mitogen-activated protein kinase kinase 4)

Genes (Homo sapiens)
BRMS1  (BRMS1 transcriptional repressor and anoikis regulator)
CD82  (CD82 molecule)
KISS1  (KiSS-1 metastasis suppressor)
MAP2K4  (mitogen-activated protein kinase kinase 4)


Additional Information