RGD Reference Report - A novel biological function for CD44 in axon growth of retinal ganglion cells identified by a bioinformatics approach. - Rat Genome Database

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A novel biological function for CD44 in axon growth of retinal ganglion cells identified by a bioinformatics approach.

Authors: Ries, A  Goldberg, JL  Grimpe, B 
Citation: Ries A, etal., J Neurochem. 2007 Nov;103(4):1491-505. Epub 2007 Aug 30.
RGD ID: 2289365
Pubmed: PMID:17760872   (View Abstract at PubMed)
PMCID: PMC2901540   (View Article at PubMed Central)
DOI: DOI:10.1111/j.1471-4159.2007.04858.x   (Journal Full-text)

The failure of CNS regeneration and subsequent motor and sensory loss remain major unsolved questions despite massive accumulation of experimental observations and results. The sheer volume of data and the variety of resources from which these data are generated make it difficult to integrate prior work to build new hypotheses. To address these challenges we developed a prototypic suite of computer programs to extract protein names from relevant publications and databases and associated each of them with several general categories of biological functions in nerve regeneration. To illustrate the usefulness of our data mining approach, we utilized the program output to generate a hypothesis for a biological function of CD44 interaction with osteopontin (OPN) and laminin in axon outgrowth of CNS neurons. We identified CD44 expression in retinal ganglion cells and when these neurons were plated on poly-l-lysine 3% of them initiated axon growth, on OPN 15%, on laminin-111 (1x) 41%, on laminin-111 (0.5x) 56%, and on a mixture of OPN and laminin (1x) 67% of neurons generated axon growth. With the aid of a deoxyribozyme (DNA enzyme) to CD44 that digests the target mRNA, we demonstrated that a reduction of CD44 expression led to reduced axon initiation of retinal ganglion cells on all substrates. We suggest that such an integrative, applied systems biology approach to CNS trauma will be critical to understand and ultimately overcome the failure of CNS regeneration.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuron projection development  IMP 2289365 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cd44  (CD44 molecule)


Additional Information