RGD Reference Report - Regulation of GABA transporter mRNA and protein after photothrombotic infarct in rat brain. - Rat Genome Database

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Regulation of GABA transporter mRNA and protein after photothrombotic infarct in rat brain.

Authors: Frahm, C  Haupt, C  Weinandy, F  Siegel, G  Bruehl, C  Witte, OW 
Citation: Frahm C, etal., J Comp Neurol. 2004 Oct 11;478(2):176-88.
RGD ID: 1643205
Pubmed: PMID:15349978   (View Abstract at PubMed)
DOI: DOI:10.1002/cne.20282   (Journal Full-text)

Animal models of focal ischemic infarcts reveal an impaired GABAergic (gamma-aminobutyric acid) neurotransmission. GABA, the main inhibitory neurotransmitter, is primarily taken up by specific sodium-dependent transporters. As these transporters play a crucial role in maintaining levels of GABA concentration, they may be functionally involved in ischemic processes. We investigated whether the mRNA and protein expression of GAT-1, the dominant neuronal GABA transporter, is altered after cortical infarct induced by photothrombosis in Wistar rats. In situ hybridization was performed to analyze GAT-1 mRNA-positive cells in cortical brain regions and the hippocampus. The lesion dramatically raised the number of GABA transporter mRNA-expressing cells in all investigated cortical regions. Double-labeling studies with a general neuronal marker and a marker for astrocytes revealed that cells expressing GAT-1 mRNA after photothrombosis are neurons. The mRNA expression pattern of all hippocampal subfields remained unchanged. In contrast, cortical GAT-1 protein density was only slightly affected and surprisingly in the opposite way. In the primary and secondary somatosensory cortex, density values were significantly reduced. Immunoreactivity was not altered in all investigated hippocampal areas. We found a marked discordance between the increased number of cells expressing GAT-1 mRNA in the cortex and the reduced tissue GAT-1 protein content. Focal brain ischemia obviously triggers mechanisms that interfere with GAT-1 transcriptional regulation and protein synthesis or turnover.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cerebral infarction  ISOSlc6a1 (Rattus norvegicus)1643205; 1643205mRNA:increased expression:cerebral cortex and neuronRGD 
cerebral infarction  IEP 1643205mRNA:increased expression:cerebral cortex and neuronRGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc6a1  (solute carrier family 6 member 1)

Genes (Mus musculus)
Slc6a1  (solute carrier family 6 (neurotransmitter transporter, GABA), member 1)

Genes (Homo sapiens)
SLC6A1  (solute carrier family 6 member 1)


Additional Information