RGD Reference Report - Identification of novel differentially expressed genes by the effect of a high-fat n-6 diet in experimental breast cancer. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Identification of novel differentially expressed genes by the effect of a high-fat n-6 diet in experimental breast cancer.

Authors: Escrich, E  Moral, R  Garcia, G  Costa, I  Sanchez, JA  Solanas, M 
Citation: Escrich E, etal., Mol Carcinog. 2004 Jun;40(2):73-8.
RGD ID: 1642758
Pubmed: PMID:15170812   (View Abstract at PubMed)
DOI: DOI:10.1002/mc.20028   (Journal Full-text)

In previous studies, we demonstrated that high corn oil diets promote the development of 7,12-dimethylbenz(alpha)anthracene (DMBA)-induced mammary tumors. In this study, we have investigated whether modulation of gene expression is one of the mechanisms by which this high-fat diet exerts such effects. Female Sprague-Dawley rats were induced with DMBA and fed normolipidic (3% corn oil) or high-fat (20% corn oil) diet. Screening of genes differentially expressed in adenocarcinomas from the high corn oil diet group compared to the control diet group was performed with cDNA microarrays. The resulting six upregulated and nine downregulated genes were validated by Northern blot and/or reverse transcription (RT)-polymerase chain reaction (PCR). Further investigation in a higher number of adenocarcinomas showed that in the high-fat n-6 diet group, where the tumor phenotype was verified to be more aggressive, the expression of submaxillary gland alpha-2u globulin, vitamin D(3)-upregulated protein 1 (VDUP1), H19, and the unknown function gene that codifies the expressed sequence tag (EST)-Rn.32385 was significantly decreased in comparison with the control group (C). These results, together with the fact that VDUP1, H19, and this globulin have been associated with cell proliferation and differentiation, open a new line of research about how the underexpression of these genes contributes to the stimulating effect of a high corn oil diet on experimental mammary carcinogenesis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Mammary Neoplasms  IEP 1642758 RGD 
Experimental Mammary Neoplasms  ISOH19 (Rattus norvegicus)1642758; 1642758 RGD 
Experimental Mammary Neoplasms severityISOTxnip (Rattus norvegicus)1642758; 1642758mRNA:decreased expression:mammary glandRGD 
Experimental Mammary Neoplasms severityIEP 1642758mRNA:decreased expression:mammary glandRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to lipid  IEP 1642758 RGD 

Objects Annotated

Genes (Rattus norvegicus)
H19  (H19 imprinted maternally expressed transcript)
Txnip  (thioredoxin interacting protein)

Genes (Mus musculus)
H19  (H19, imprinted maternally expressed transcript)
Txnip  (thioredoxin interacting protein)

Genes (Homo sapiens)
H19  (H19 imprinted maternally expressed transcript)
TXNIP  (thioredoxin interacting protein)


Additional Information