RGD Reference Report - Rat duodenal IRP1 activity and iron absorption in iron deficiency and after HO perfusion. - Rat Genome Database

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Rat duodenal IRP1 activity and iron absorption in iron deficiency and after HO perfusion.

Authors: Schumann, K  Brennan, K  Weiss, M  Pantopoulos, K  Hentze, MW 
Citation: Schumann K, etal., Eur J Clin Invest. 2004 Apr;34(4):275-82.
RGD ID: 1642737
Pubmed: PMID:15086359   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1365-2362.2004.01335.x   (Journal Full-text)

BACKGROUND: Iron regulatory protein 1 (IRP1), a post-transcriptional regulator of iron metabolism, is activated in the duodenum of iron-deficient animals, which is associated with increased iron absorption. In cell cultures IRP1 was also activated by iron-independent signals, such as H(2)O(2). Here we investigate whether luminal perfusion of rat duodenum with H(2)O(2) activates duodenal IRP1 and modulates duodenal iron absorption. METHODS: Duodena from iron-adequate Sprague-Dawley rats were luminally perfused with H(2)O(2). Iron regulatory protein-1 activity was determined in duodenal mucosa or in villus and crypt preparations by an electrophoretic mobility shift assay. Duodenal (59)Fe absorption was measured in isolated, perfused duodenal segments ex vivo and in ligated loops in vivo. (59)Fe uptake from the blood side was assessed after i.v. injection of (59)Fe-nitrilotriacetic acid. RESULTS: Similar to iron deficiency, the perfusion with 0-50 mM of H(2)O(2) increases duodenal IRP1 activity along the entire crypt villus-axis in a dose-dependent manner. After H(2)O(2) treatment, IRP1 remains activated for 12-24 h in the tips and for 72 h in the crypts. In iron-deficiency, IRP activation correlates with increased (59)Fe absorption. However, the H(2)O(2) treatment fails to stimulate any increase in (59)Fe uptake, without promoting damage of mucosal architecture or impairing glucose and water transport. CONCLUSION: Duodenal (59)Fe uptake is not affected by the H(2)O(2)-mediated activation of IRP1.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
intracellular iron ion homeostasis  IDA 1642737 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
iron-responsive element binding  IDA 1642737 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aco1  (aconitase 1)


Additional Information