RGD Reference Report - Methylmercury stimulates arachidonic acid release and cytosolic phospholipase A2 expression in primary neuronal cultures. - Rat Genome Database

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Methylmercury stimulates arachidonic acid release and cytosolic phospholipase A2 expression in primary neuronal cultures.

Authors: Shanker, G  Hampson, RE  Aschner, M 
Citation: Shanker G, etal., Neurotoxicology. 2004 Mar;25(3):399-406.
RGD ID: 1642468
Pubmed: PMID:15019302   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuro.2003.08.008   (Journal Full-text)

Cytosolic phospholipase A2 (cPLA2) plays an important role in the stimulus-dependent hydrolysis of sn-2 ester bond from membrane phospholipids, releasing arachidonic acid (AA), which along with its metabolites is involved in a number of regulatory functions. The present study examined the effect of methylmercury (MeHg; 0, 2.5, 5.0 microM) on cPLA2 activation in primary hippocampal neurons by assessing the release of 3H-AA. A significant increase in AA release was observed in cultures treated with 5 microM MeHg (10, 30, 60 and 120 min). This effect was due to neuronal cPLA2 activation, since it was completely abolished by arachidonyl trifluoromethyl ketone (AACOCF3), a specific inhibitor of cPLA2. Additional studies confirmed, by means of western blot analysis, that MeHg (5.0 and 10 microM; 16h) potently increases neuronal cPLA2 protein expression. These results suggest that cPLA2-stimulated hydrolysis and release of AA are potential mediators of MeHg-induced neurotoxicity.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to methylmercury  IEP 1642468 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pla2g4a  (phospholipase A2 group IVA)


Additional Information