RGD Reference Report - Characterization of interactions between Nedd4 and beta and gammaENaC using surface plasmon resonance. - Rat Genome Database

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Characterization of interactions between Nedd4 and beta and gammaENaC using surface plasmon resonance.

Authors: Asher, C  Chigaev, A  Garty, H 
Citation: Asher C, etal., Biochem Biophys Res Commun. 2001 Sep 7;286(5):1228-31.
RGD ID: 1642065
Pubmed: PMID:11527431   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2001.5508   (Journal Full-text)

Cell surface expression of the epithelial Na(+) channel ENaC is regulated by the ubiquitin ligase Nedd4. Binding of the WW domains of Nedd4 to the PY region in the carboxy tails of beta and gammaENaC, results in channel ubiquitination and degradation. Kinetic analysis of these interactions has been done using surface plasmon resonance. Synthetic peptides corresponding to the PY regions of beta and gammaENaC were immobilized on a sensor chip and "real-time" kinetics of their binding to recombinant WW proteins was determined. Specificity of the interactions was established by competition experiment, as well as by monitoring effects of a point mutation known to impair Nedd4/ENaC binding. These data provides the first determination of association, dissociation and equilibrium constants for the interactions between WW2 and beta or gammaENaC.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Nedd4  (NEDD4 E3 ubiquitin protein ligase)
Scnn1b  (sodium channel epithelial 1 subunit beta)
Scnn1g  (sodium channel epithelial 1 subunit gamma)


Additional Information