RGD Reference Report - Effects of apolipoproteins A-IV and A-I on the uptake of phospholipid liposomes by hepatocytes. - Rat Genome Database

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Effects of apolipoproteins A-IV and A-I on the uptake of phospholipid liposomes by hepatocytes.

Authors: Bisgaier, CL  Siebenkas, MV  Williams, KJ 
Citation: Bisgaier CL, etal., J Biol Chem. 1989 Jan 15;264(2):862-6.
RGD ID: 1626397
Pubmed: PMID:2492020   (View Abstract at PubMed)

We examined the effects of apolipoproteins A-IV and A-I on the catabolism of whole particles by hepatoma G2 cells and cultured primary hepatocytes. For this type of experiment, high density lipoprotein is unsuitable, because all of its lipid and protein components independently dissociate and exchange and hence poorly trace whole particle catabolism. We therefore used phosphatidylcholine liposomes with radioactive tracers entrapped within their aqueous cores. Apolipoproteins A-IV, A-I, or E added to liposomes became liposome-associated and produced no detectable release of encapsulated label. As a positive control, apolipoprotein E doubled the uptake of labeled liposomes by hepatoma cells, compared to apolipoprotein-free controls, and this increase could be blocked by the addition of excess unlabeled low density lipoprotein. Degradation of labeled liposomes by hepatoma cells was increased 6-fold by the addition of apolipoprotein E. In contrast, neither apolipoprotein A-IV nor A-I increased cellular uptake or degradation of the particles. Similar results were obtained with primary hepatocytes. In studies using apolipoprotein combinations, apolipoproteins A-IV and A-I were each able to displace apolipoprotein E from liposomes and thereby reduce cellular uptake. Our data indicate that apolipoproteins A-IV and A-I do not facilitate uptake or degradation of whole particles by liver-derived cells in vitro. However, these apolipoproteins may modulate receptor-mediated uptake of particles by reducing the amount of particle-bound apolipoprotein E.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lipid transport  IDA 1626397 RGD 
lipoprotein metabolic process  IDA 1626397 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lipid transporter activity  IDA 1626397 RGD 
phospholipid binding  IDA 1626397 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apoe  (apolipoprotein E)


Additional Information