RGD Reference Report - Corticotropin-releasing factor receptor types 1 and 2 are differentially expressed in pre- and post-synaptic elements in the post-natal developing rat cerebellum. - Rat Genome Database

RGD Reference Report - Corticotropin-releasing factor receptor types 1 and 2 are differentially expressed in pre- and post-synaptic elements in the post-natal developing rat cerebellum. - Rat Genome Database

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General

Corticotropin-releasing factor receptor types 1 and 2 are differentially expressed in pre- and post-synaptic elements in the post-natal developing rat cerebellum.
Authors:	Swinny, JD Kalicharan, D Blaauw, EH Ijkema-Paassen, J Shi, F Gramsbergen, A Van der Want, JJ
Citation:	Swinny JD, etal., Eur J Neurosci. 2003 Aug;18(3):549-62.
RGD ID:	1626245
Pubmed:	PMID:12911751 (View Abstract at PubMed)
Corticotropin-releasing factor (CRF)-like proteins act via two G-protein-coupled receptors (CRF-R1 and CRF-R2) playing important neuromodulatory roles in stress responses and synaptic plasticity. The cerebellar expression of corticotropin-releasing factor-like ligands has been well documented, but their receptor localization has not. This is the first combination of a light microscopic and ultrastructural study to localize corticotropin-releasing factor receptors immunohistologically in the developing rat cerebellum. Both CRF-R1 and CRF-R2 were expressed in climbing fibres from early stages (post-natal day 3) to the adult, but CRF-R2 immunoreactivity was only prominent throughout the molecular layer in the posterior cerebellar lobules. CRF-R1 immunoreactivity was concentrated in apical regions of Purkinje cell somata and later in primary dendrites exhibiting a diffuse cytoplasmic appearance. In Purkinje cells, CRF-R1 immunoreactivity was never membrane bound post-synaptically in dendritic spines while CRF-R2 immunoreactivity was found on plasmic membranes of Purkinje cells from post-natal day 15 onwards. We conclude that the localization of these receptors in cerebellar afferents implies their pre-synaptic control of the release of corticotropin-releasing factor-like ligands, impacting on the sensory information being transmitted from afferents. Furthermore, the fact that CRF-R2 is membrane bound at synapses, while CRF-R1 is not, suggests that ligands couple to CRF-R2 via synaptic transmission and to CRF-R1 via volume transmission. Finally, the distinct expression profiles of receptors along structural domains of Purkinje cells suggest that the role for these receptors is to modulate afferent inputs.

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Cellular Component

Only show annotations with direct experimental evidence (0 objects hidden)

Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
apical part of cell		IDA		1626245	Purkinje cells beginning at postnatal day 6	RGD
dendrite		IDA		1626245	Purkinje cells beginning at postnatal day 6	RGD
dendritic spine	NOT	IDA		1626245	Purkinje cells	RGD
multivesicular body		IDA		1626245	Purkinje cells beginning postnatal day 3	RGD
neuronal cell body		IDA		1626245	Purkinje cells beginning postnatal day 3	RGD
trans-Golgi network		IDA		1626245	Purkinje cells beginning postnatal day 3	RGD
vesicle		IDA		1626245	Purkinje cells beginning postnatal day 3	RGD

Objects Annotated

Genes (Rattus norvegicus)

Crhr1 (corticotropin releasing hormone receptor 1)

Additional Information