RGD Reference Report - KATP channel conductance of descending vasa recta pericytes. - Rat Genome Database

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KATP channel conductance of descending vasa recta pericytes.

Authors: Cao, C  Lee-Kwon, W  Silldorff, EP  Pallone, TL 
Citation: Cao C, etal., Am J Physiol Renal Physiol. 2005 Dec;289(6):F1235-45. Epub 2005 Jul 26.
RGD ID: 1625261
Pubmed: PMID:16048905   (View Abstract at PubMed)
DOI: DOI:10.1152/ajprenal.00111.2005   (Journal Full-text)

Using nystatin-perforated patch-clamp and whole cell recording, we tested the hypothesis that K(ATP) channels contribute to resting conductance of rat descending vasa recta (DVR) pericytes and are modulated by vasoconstrictors. The K(ATP) blocker glybenclamide (Glb; 10 microM) depolarized pericytes and inhibited outward currents of cells held at -40 mV. K(ATP) openers pinacidil (Pnc; 10 microM) and P-1075 (1 microM) hyperpolarized pericytes and transiently augmented outward currents. All effects of Pnc and P-1075 were fully reversed by Glb. Inward currents of pericytes held at -60 mV in symmetrical 140 mM K(+) were markedly augmented by Pnc and fully reversed by Glb. Ramp depolarizations in symmetrical K(+), performed in Pnc and Pnc + Glb, yielded a Pnc-induced, Glb-sensitive K(ATP) difference current that lacked rectification and reversed at 0 mV. Immunostaining identified both K(IR)6.1, K(IR)6.2 inward rectifier subunits and sulfonurea receptor subtype 2B. ANG II (1 and 10 nM) and endothelin-1 (10 nM) but not vasopressin (100 nM) significantly lowered holding current at -40 mV and abolished Pnc-stimulated outward currents. We conclude that DVR pericytes express K(ATP) channels that make a significant contribution to basal K(+) conductance and are inhibited by ANG II and endothelin-1.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
potassium ion transport  IMP 1625261; 1625261 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnj11  (potassium inwardly-rectifying channel, subfamily J, member 11)
Kcnj8  (potassium inwardly-rectifying channel, subfamily J, member 8)


Additional Information