RGD Reference Report - Subcellular distributions of adenosine A1 and A2A receptors in the rat dorsomedial nucleus of the solitary tract at the level of the area postrema. - Rat Genome Database

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Subcellular distributions of adenosine A1 and A2A receptors in the rat dorsomedial nucleus of the solitary tract at the level of the area postrema.

Authors: Pickel, VM  Chan, J  Linden, J  Rosin, DL 
Citation: Pickel VM, etal., Synapse. 2006 Dec 1;60(7):496-509.
RGD ID: 1625217
Pubmed: PMID:16952160   (View Abstract at PubMed)
DOI: DOI:10.1002/syn.20326   (Journal Full-text)

Adenosine A1 and A2A receptors mediate distinct cardiovascular components of defense reactions that are ascribed, in part, to opposing actions within the nucleus tractus solitarius. To assess the cellular sites of relevance to these actions, we examined the light and electron microscopic immunolabeling of adenosine A1 and A2A receptors in the rat dorsomedial nucleus of the solitary tract at the level of the area postrema (dmNTS-AP), a region crucial for cardiovascular regulation involving vagal baroreceptor afferents. Immunoreactivity for each receptor was independently localized to distinct segments of plasma membranes and endomembranes in somatodendritic, axonal, and glial profiles. The dendritic labeling for each receptor also was detected within and near asymmetric, excitatory-type synapses. Of all peroxidase labeled profiles exclusive of somata, approximately 58% were A1- and 39% were A2A-labeled dendrites. Dendrites and astrocytic glia were the profiles that most often expressed both subtypes of adenosine receptors. The axonal labeling for A2A receptors was seen mainly in unmyelinated axons, whereas the A1 receptors were prominently localized within axon terminals. These terminals often formed single or multisynaptic excitatory-type junctions or single symmetric synapses on dendrites, a few of which expressed A1 and A2A receptors. These results provide the first ultrastructural evidence that A1 and A2A receptors have distributions conductive to their dual involvement in modulating the output of single neurons and glial function in the dmNTS-AP, where the predominate presynaptic effects of adenosine are mediated through A1 receptors.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axon  IDA 1625217 RGD 
dendrite  IDA 1625217; 1625217 RGD 
postsynaptic membrane is_active_inIDA 1625217; 1625217PMID:16952160SynGO 
postsynaptic membrane located_inIDA 1625217; 1625217PMID:16952160SynGO-UCL 
presynaptic membrane is_active_inIDA 1625217; 1625217PMID:16952160SynGO 
presynaptic membrane located_inIDA 1625217; 1625217PMID:16952160SynGO-UCL 
terminal bouton  IDA 1625217 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Adora1  (adenosine A1 receptor)
Adora2a  (adenosine A2a receptor)


Additional Information