RGD Reference Report - Endocrine pancreatic tissue plasticity in obese humans is associated with cytoplasmic expression of PBX-1 in pancreatic ductal cells. - Rat Genome Database

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Endocrine pancreatic tissue plasticity in obese humans is associated with cytoplasmic expression of PBX-1 in pancreatic ductal cells.

Authors: Muharram, G  Beucher, A  Moerman, E  Belaich, S  Gmyr, V  Vandewalle, B  Pattou, F  Kerr-Conte, J 
Citation: Muharram G, etal., Biochem Biophys Res Commun. 2005 Aug 12;333(4):1153-9.
RGD ID: 1625044
Pubmed: PMID:15979049   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2005.05.199   (Journal Full-text)

In vivo lineage tracing experiments in mice have recently cast doubt on the potential islet neogenesis from ductal precursors in adult mammals. We examined, in human obesity, a model for pancreatic endocrine tissue plasticity, the gene and protein expression of PBX-1-a transcription factor expressed in regenerating rat ductules and potentially implicated in the pancreatic development, alone or in association with PDX-1. When comparing gene expression, by quantitative real-time RT-PCR, in pancreatic exocrine tissue from obese non-diabetic subjects with increased islet mass, we found that Pbx-1 and Pdx-1 were up-regulated (5.9+/-1.2 and 2.4+/-0.6 versus non-obese). Immunohistochemistry confirmed PBX-1 over-expression and its cytoplasmic sequestration in ductal cells of obese subjects, associated with pronounced islet neogenesis (cytokeratin 19/chromogranin A double labeling). cDNA microarray analysis also showed up-regulation of other genes implicated in islet regeneration, including betacellulin, laminin, TGFa, NeuroD1, Pax6, substantiating the role of the islet neogenesis pathway in human obesity.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
obesity  IEP 1625044mRNA:increased expression:pancreasRGD 
obesity  ISONEUROD1 (Homo sapiens)1625044; 1625044mRNA:increased expression:pancreasRGD 
obesity  IEP 1625044mRNA:increased expression:exocrine pancreas (human)RGD 
obesity  ISOPDX1 (Homo sapiens)1625044; 1625044mRNA:increased expression:exocrine pancreas (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Neurod1  (neuronal differentiation 1)
Pdx1  (pancreatic and duodenal homeobox 1)

Genes (Mus musculus)
Neurod1  (neurogenic differentiation 1)
Pdx1  (pancreatic and duodenal homeobox 1)

Genes (Homo sapiens)
NEUROD1  (neuronal differentiation 1)
PDX1  (pancreatic and duodenal homeobox 1)


Additional Information