RGD Reference Report - Direct protein-protein interaction between PLCgamma1 and the bradykinin B2 receptor--importance of growth conditions. - Rat Genome Database

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Direct protein-protein interaction between PLCgamma1 and the bradykinin B2 receptor--importance of growth conditions.

Authors: Duchene, J  Chauhan, SD  Lopez, F  Pecher, C  Esteve, JP  Girolami, JP  Bascands, JL  Schanstra, JP 
Citation: Duchene J, etal., Biochem Biophys Res Commun. 2005 Jan 28;326(4):894-900.
RGD ID: 1625041
Pubmed: PMID:15607753   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2004.11.126   (Journal Full-text)

Recently, we have described a novel protein-protein interaction between the G-protein coupled bradykinin B2 receptor and tyrosine phosphatase SHP-2 via an immunoreceptor tyrosine-based inhibition motif (ITIM) sequence located in the C-terminal part of the B2 receptor and the Src homology (SH2) domains of SHP-2. Here we show that phospholipase C (PLC)gamma1, another SH2 domain containing protein, can also interact with this ITIM sequence. Using surface plasmon resonance analysis, we observed that PLCgamma1 interacted with a peptide containing the phosphorylated form of the bradykinin B2 receptor ITIM sequence. In CHO cells expressing the wild-type B2 receptor, bradykinin-induced transient recruitment and activation of PLCgamma1. Interestingly, this interaction was only observed in quiescent and not in proliferating cells. Mutation of the key ITIM residue abolished this interaction with and activation of PLCgamma1. Finally we also identified bradykinin-induced PLCgamma1 recruitment and activation in primary culture renal mesangial cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIPlcg1 (Rattus norvegicus)1625041 RGD 
protein binding  IPIBdkrb2 (Rattus norvegicus)1625041 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bdkrb2  (bradykinin receptor B2)
Plcg1  (phospholipase C, gamma 1)


Additional Information