RGD Reference Report - Increased expression of angiopoietin-2 and Tie2 receptor in a rat model of myocardial ischaemia/reperfusion. - Rat Genome Database

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Increased expression of angiopoietin-2 and Tie2 receptor in a rat model of myocardial ischaemia/reperfusion.

Authors: Shyu, KG  Chang, CC  Wang, BW  Kuan, P  Chang, H 
Citation: Shyu KG, etal., Clin Sci (Lond). 2003 Sep;105(3):287-94.
RGD ID: 1601505
Pubmed: PMID:12737621   (View Abstract at PubMed)
DOI: DOI:10.1042/CS20030025   (Journal Full-text)

The angiopoietins and Tie receptors are involved in blood vessel formation. The role of the angiopoietin/Tie receptor system in myocardial ischaemia/reperfusion is not well known. To investigate the participation of angiopoietins and Tie receptors in myocardial ischaemia/reperfusion, adult Wistar rats were studied in which the left coronary artery was ligated for 30 min, followed by reperfusion. Angiopoietin-1 (Ang1), angiopoietin-2 (Ang2), Tie1 and Tie2 were measured immediately after relief of occlusion, and 1, 6, 24 and 72 h after reperfusion, by Northern blot, Western blot and immunohistochemical staining. Ang2 mRNA was increased significantly at 24 h and 48 h after reperfusion, and returned to baseline levels at 72 h, in the jeopardized myocardium. Tie2 mRNA increased 3.4-fold immediately after the relief of occlusion, reached a maximum 8-fold increase at 24 h after reperfusion and remained elevated up to 72 h. Ang2 protein levels also increased after reperfusion, reaching a maximum 2.2-fold increase at 48 h after reperfusion. Tie2 protein increased immediately after relief of ischaemia, and showed a significant increase from 6 h to 72 h after reperfusion as compared with the sham control. Ang1 and Tie1 mRNA and protein did not show significant changes after ischaemia/reperfusion. Immunohistochemical studies also showed increased immunoreactivity of Ang2 and Tie2 in the jeopardized myocardium after ischaemia/reperfusion. In conclusion, expression of both Ang2 and Tie2 increased after ischaemia/reperfusion in the rat ventricular myocardium, while the expression of Ang1 and Tie1 did not.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Reperfusion Injury  ISOAngpt2 (Rattus norvegicus)1601505; 1601505mRNA and protein:increased expression:myocardium (rat)RGD 
Myocardial Reperfusion Injury  IEP 1601505mRNA and protein:increased expression:myocardium (rat)RGD 
Myocardial Reperfusion Injury  ISOTek (Rattus norvegicus)1601505; 1601505mRNA and protein:increased expression:ventricle myocardiumRGD 
Myocardial Reperfusion Injury  IEP 1601505mRNA and protein:increased expression:ventricle myocardiumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Angpt2  (angiopoietin 2)
Tek  (TEK receptor tyrosine kinase)

Genes (Mus musculus)
Angpt2  (angiopoietin 2)
Tek  (TEK receptor tyrosine kinase)

Genes (Homo sapiens)
ANGPT2  (angiopoietin 2)
TEK  (TEK receptor tyrosine kinase)


Additional Information