RGD Reference Report - MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin. - Rat Genome Database

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MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin.

Authors: Nan, X  Campoy, FJ  Bird, A 
Citation: Nan X, etal., Cell. 1997 Feb 21;88(4):471-81.
RGD ID: 1601325
Pubmed: PMID:9038338   (View Abstract at PubMed)

MeCP2 is an abundant mammalian protein that binds to methylated CpG. We have found that native and recombinant MeCP2 repress transcription in vitro from methylated promoters but do not repress nonmethylated promoters. Repression is nonlinearly dependent on the local density of methylation, becoming significant at the density found in bulk vertebrate genomic DNA. Transient transfection using fusions with the GAL4 DNA binding domain identified a region of MeCP2 that is capable of long-range repression in vivo. Moreover, MeCP2 is able to displace histone H1 from preassembled chromatin that contains methyl-CpG. These properties, together with the abundance of MeCP2 and the high frequency of its 2 bp binding site, suggest a role as a global transcriptional repressor in vertebrate genomes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of DNA-templated transcription  IDA 1601325 RGD 
negative regulation of transcription by RNA polymerase II  IDA 1601325 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
chromatin  IDA 1601325 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
chromatin DNA binding  IDA 1601325 RGD 
double-stranded methylated DNA binding  IDA 1601325 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mecp2  (methyl CpG binding protein 2)


Additional Information