RGD Reference Report - Orexin expression and function: glucocorticoid manipulation, stress, and feeding studies. - Rat Genome Database

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Orexin expression and function: glucocorticoid manipulation, stress, and feeding studies.

Authors: Ford, GK  Al-Barazanji, KA  Wilson, S  Jones, DN  Harbuz, MS  Jessop, DS 
Citation: Ford GK, etal., Endocrinology. 2005 Sep;146(9):3724-31. Epub 2005 Jun 16.
RGD ID: 1600976
Pubmed: PMID:15961555   (View Abstract at PubMed)
DOI: DOI:10.1210/en.2005-0496   (Journal Full-text)

We investigated the effects of glucocorticoid manipulation on orexin-A-induced feeding and prepro-orexin mRNA levels in the lateral hypothalamic area (LHA) of the rat brain. Adrenalectomy (ADX) reduced orexin-A-induced feeding over 4 h by about 60%, compared with shams, an effect that was reversed by corticosterone (CORT) replacement. ADX had no effect on prepro-orexin mRNA levels in the LHA in either the morning or the evening; however, message was up-regulated by CORT in the morning but not the evening. An increased number of emulsion grains per cell in the LHA suggests that this is a specific increase in prepro-orexin mRNA and is not due to an increased number of cells expressing message. Prepro-orexin mRNA levels in the LHA were elevated 4 h after injection of lipopolysaccharide, compared with saline-injected controls. Partial but not complete abolition of orexin-A-induced feeding by ADX suggests that orexin-A-induced feeding may be mediated through glucocorticoid-dependent and glucocorticoid-independent pathways. In the morning increased prepro-orexin mRNA after CORT replacement demonstrates that orexin expression is sensitive to increased concentrations of glucocorticoids. However, the lack of effect of ADX on prepro-orexin mRNA levels suggests that endogenous glucocorticoids are not involved in tonic regulation of basal prepro-orexin expression. Overall our data constitute a body of evidence for an integrated relationship between central orexin expression, stress, glucocorticoid manipulation, and feeding patterns in the rat.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
eating behavior  IDA 1600976 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hcrt  (hypocretin neuropeptide precursor)


Additional Information