RGD Reference Report - Intra-acinar cell activation of trypsinogen during caerulein-induced pancreatitis in rats. - Rat Genome Database

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Intra-acinar cell activation of trypsinogen during caerulein-induced pancreatitis in rats.

Authors: Hofbauer, B  Saluja, AK  Lerch, MM  Bhagat, L  Bhatia, M  Lee, HS  Frossard, JL  Adler, G  Steer, ML 
Citation: Hofbauer B, etal., Am J Physiol. 1998 Aug;275(2 Pt 1):G352-62.
RGD ID: 1599965
Pubmed: PMID:9688663   (View Abstract at PubMed)

Supramaximal stimulation of the pancreas with the CCK analog caerulein causes acute edematous pancreatitis. In this model, active trypsin can be detected in the pancreas shortly after the start of supramaximal stimulation. Incubation of pancreatic acini in vitro with a supramaximally stimulating caerulein concentration also results in rapid activation of trypsinogen. In the current study, we have used the techniques of subcellular fractionation and both light and electron microscopy immunolocalization to identify the site of trypsinogen activation and the subsequent fate of trypsin during caerulein-induced pancreatitis. We report that trypsin activity and trypsinogen-activation peptide (TAP), which is released on activation of trypsinogen, are first detectable in a heavy subcellular fraction. This fraction is enriched in digestive enzyme zymogens and lysosomal hydrolases. Subsequent to trypsinogen activation, both trypsin activity and TAP move to a soluble compartment. Immunolocalization studies indicate that trypsinogen activation occurs in cytoplasmic vacuoles that contain the lysosomal hydrolase cathepsin B. These observations suggest that, during the early stages of pancreatitis, trypsinogen is activated in subcellular organelles containing colocalized digestive enzyme zymogens and lysosomal hydrolases and that, subsequent to its activation, trypsin is released into the cytosol.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
pancreatitis  ISOPrss1 (Rattus norvegicus)1599965; 1599965protein:increased expression:cytosolRGD 
pancreatitis  IEP 1599965protein:increased expression:cytosolRGD 

Objects Annotated

Genes (Rattus norvegicus)
Prss1  (serine protease 1)

Genes (Mus musculus)
Prss1  (serine protease 1 (trypsin 1))

Genes (Homo sapiens)
PRSS1  (serine protease 1)


Additional Information