RGD Reference Report - Apoptosis and its pathway in early post-implantation embryos of diabetic rats. - Rat Genome Database

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Apoptosis and its pathway in early post-implantation embryos of diabetic rats.

Authors: Sun, F  Kawasaki, E  Akazawa, S  Hishikawa, Y  Sugahara, K  Kamihira, S  Koji, T  Eguchi, K 
Citation: Sun F, etal., Diabetes Res Clin Pract. 2005 Feb;67(2):110-8.
RGD ID: 1599491
Pubmed: PMID:15649569   (View Abstract at PubMed)
DOI: DOI:10.1016/j.diabres.2004.06.008   (Journal Full-text)

It has been reported that diabetes-induced inappropriate apoptosis in embryos during neurulation may be one of the mechanisms leading to neural tube defects. We studied apoptosis and the apoptotic pathway occurring in early post-implantation period embryos of non-diabetic and streptozotocin (STZ)-induced diabetic rats. In quantitative RT-PCR, bax mRNA was constantly expressed to similar degree in embryos of non-diabetic and diabetic rats, while the expression of bcl-2 mRNA was significantly decreased in diabetic rat embryos compared to non-diabetic rat embryos. The increased number of terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL)-positive cells occurred selectively in the primitive brains of diabetic rat embryos compared to non-diabetic rat embryos. Immunohistochemical studies revealed that, in mirror sections, the staining of Bax and activated caspase-3 were observed in the TUNEL-positive cell area, but the expression of Bcl-2 in these apoptotic cells was generally too low to be detected. These results suggest that a Bax-regulated mitochondrial cytochrome c-mediated caspase-3 activation pathway might be involved in the diabetic embryopathy.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Diabetes Mellitus  ISOBcl2 (Rattus norvegicus)1599491; 1599491mRNA:decreased expression:embryoRGD 
Experimental Diabetes Mellitus  IEP 1599491mRNA:decreased expression:embryoRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bcl2  (BCL2, apoptosis regulator)

Genes (Mus musculus)
Bcl2  (B cell leukemia/lymphoma 2)

Genes (Homo sapiens)
BCL2  (BCL2 apoptosis regulator)


Additional Information