RGD Reference Report - Differences in myofilament calcium sensitivity in rat psoas fibers reconstituted with troponin T isoforms containing the alpha- and beta-exons. - Rat Genome Database

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Differences in myofilament calcium sensitivity in rat psoas fibers reconstituted with troponin T isoforms containing the alpha- and beta-exons.

Authors: Gallon, CE  Tschirgi, ML  Chandra, M 
Citation: Gallon CE, etal., Arch Biochem Biophys. 2006 Dec 15;456(2):127-34. Epub 2006 Jun 22.
RGD ID: 1599483
Pubmed: PMID:16839517   (View Abstract at PubMed)
DOI: DOI:10.1016/j.abb.2006.06.008   (Journal Full-text)

The carboxy terminus of fast skeletal muscle troponin T (fsTnT) is highly conserved. However, mutually exclusive splicing of exons 16 and 17 in the fsTnT gene results in the expression of either the alpha- or beta-fsTnT isoform. The alpha-isoform is expressed only in adult fast skeletal muscle, whereas the beta-isoform is expressed in varying quantities throughout muscle development. Reconstitution of detergent-skinned adult rat psoas muscle fibers with rat fast skeletal troponin complexes containing either fsTnT isoform demonstrated that reconstitution with alpha-fsTnT resulted in greater myofilament Ca(2+) sensitivity than reconstitution with beta-fsTnT, without changes to Ca(2+)-activated maximal tension, ATPase activity or tension cost. The observed isoform-specific differences in myofilament Ca(2+) sensitivity may be due to changes in the transition of the thin-filament regulatory unit from the off to the on state, possibly due to altered interactions of the C-terminus of fsTnT with troponins I and/or C.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
regulation of striated muscle contraction  IMP 1599483 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnnt3  (troponin T3, fast skeletal type)


Additional Information