RGD Reference Report - The effect of pH on the kinetics of arylsulphatases A and B. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

The effect of pH on the kinetics of arylsulphatases A and B.

Authors: O'Fagain, C  Butler, BM  Mantle, TJ 
Citation: O'Fagain C, etal., Biochem J. 1983 Sep 1;213(3):603-7.
RGD ID: 1599227
Pubmed: PMID:6137211   (View Abstract at PubMed)
PMCID: PMC1152174   (View Article at PubMed Central)

The effect of pH on the kinetics of rat liver arylsulphatases A and B is very similar and shows that two groups with pK values of 4.4-4.5 and 5.7-5.8 are important for enzyme activity. Substrate binding has no effect on the group with a pK of 4.4-4.5; however, the pK of the second group is shifted to 7.1-7.5 in the enzyme-substrate complex. An analysis of the effect of pH on the Ki for sulphate inhibition suggests that HSO4-is the true product. A model is proposed that involves the two ionizing groups identified in the present study in a concerted general acid-base-catalysed mechanism.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to pH  IDA 1599227; 1599227 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arsa  (arylsulfatase A)
Arsb  (arylsulfatase B)


Additional Information