RGD Reference Report - Endotoxin and cytokines alter contractile protein expression in cardiac myocytes in vivo. - Rat Genome Database

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Endotoxin and cytokines alter contractile protein expression in cardiac myocytes in vivo.

Authors: Patten, M  Kramer, E  Bunemann, J  Wenck, C  Thoenes, M  Wieland, T  Long, C 
Citation: Patten M, etal., Pflugers Arch. 2001 Sep;442(6):920-7.
RGD ID: 1598729
Pubmed: PMID:11680626   (View Abstract at PubMed)

Release of bacterial endotoxin and cytokines induce cardiac failure during sepsis. We investigated the direct effects of E. coli endotoxin (lipopolysaccharide, LPS) and cytokines induced by LPS on the cardiac myocyte gene program. For in vivo-experiments adult Wistar rats were given 600 microg/day LPS i.v. for 24 h or 7 days. In addition, cultured adult rat cardiac myocytes were treated with LPS, interleukin-1beta (IL-1beta), tumour necrosis factor-alpha (TNFalpha), interferon-gamma (IFNgamma) or IL-6 for 24 h. mRNA expression was evaluated for cardiac-alpha-actin (cAct), skeletal-alpha-actin (skAct), beta- and alpha-myosin heavy chain (MHC). LPS induced betaMHC-mRNA 3.6-fold and repressed alphaMHC 2.7-fold and cAct 2.5-fold after 24 h in vivo. Up-regulation of betaMHC (3-fold) and repression of cAct (2.5-fold) were still observed after 7 days LPS infusion, whereas alphaMHC-mRNA levels had returned to normal. At the protein level, increased expression of betaMHC by LPS treatment occurred already after 24 h and was maintained thereafter. LPS had no influence on skAct-mRNA. Similar changes in contractile protein mRNA expression were observed in LPS-treated cardiomyocytes in culture, whereas the tested cytokines either activated (IL-1beta, IFNgamma) or repressed (TNFalpha, IL-6) both MHC-isoforms and cAct. In conclusion, LPS and proinflammatory cytokines induce changes in contractile protein expression that may contribute to the acute heart failure observed during endotoxaemia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Endotoxemia  ISOActc1 (Rattus norvegicus)1598729; 1598729 RGD 
Endotoxemia  IEP 1598729 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Actc1  (actin, alpha, cardiac muscle 1)

Genes (Mus musculus)
Actc1  (actin, alpha, cardiac muscle 1)

Genes (Homo sapiens)
ACTC1  (actin alpha cardiac muscle 1)


Additional Information