RGD Reference Report - Myosin light chain isoforms modify force-generating ability of cardiac myosin by changing the kinetics of actin-myosin interaction. - Rat Genome Database

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Myosin light chain isoforms modify force-generating ability of cardiac myosin by changing the kinetics of actin-myosin interaction.

Authors: Yamashita, H  Sugiura, S  Fujita, H  Yasuda, S  Nagai, R  Saeki, Y  Sunagawa, K  Sugi, H 
Citation: Yamashita H, etal., Cardiovasc Res. 2003 Dec 1;60(3):580-8.
RGD ID: 1598726
Pubmed: PMID:14659803   (View Abstract at PubMed)

OBJECTIVE: To investigate the functional role of myosin light chain (MLC) isoforms in cardiac muscles, we examined the motor function of two different myosins the structure of which differed only in the MLC. METHODS: We purified myosin from atria (A-myosin) and ventricles (V-myosin) of young rats, which contained atrial-type and ventricular-type MLCs, respectively, but having identical alpha-heavy chain isoform. Actin filament velocity (Vel) was determined in the in vitro motility assay. Average force of myosin molecules (F) was estimated and single events of actin-myosin interaction were recorded with the laser trap technique. RESULTS: Vel was slightly higher in A-myosin than in V-myosin, while actin-activated ATPase activity was not different. F, determined from force versus actin filament length relation, was approximately 60% higher in V-myosin (3.3 vs. 2.1 pN/microm). The mean duration of isometric force events was longer in V-myosin than in A-myosin (323+/-13 vs. 294+/-30 ms, p<0.05), while the amplitudes of unitary displacement and force of a single myosin molecule did not differ between them. CONCLUSION: The MLC isoform can be a determinant of force-generating ability of cardiac myosin by modulating crossbridge kinetics without affecting the catalytic activity.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cardiac muscle contraction  IDA 1598726 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
myosin complex  IDA 1598726 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoskeletal motor activity  IDA 1598726 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Myl3  (myosin light chain 3)

Objects referenced in this article
Gene Acta1 actin, alpha 1, skeletal muscle Rattus norvegicus

Additional Information