RGD Reference Report - c-Jun N-terminal kinase (JNK) and JNK interacting protein response in rat brain after transient middle cerebral artery occlusion. - Rat Genome Database

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c-Jun N-terminal kinase (JNK) and JNK interacting protein response in rat brain after transient middle cerebral artery occlusion.

Authors: Hayashi, T  Sakai, K  Sasaki, C  Zhang, WR  Warita, H  Abe, K 
Citation: Hayashi T, etal., Neurosci Lett. 2000 Apr 28;284(3):195-9.
RGD ID: 1582317
Pubmed: PMID:10773432   (View Abstract at PubMed)

c-Jun response is involved in the development of ischemic brain injury, which is activated by c-Jun N-terminal kinase-1 (JNK-1). The activity of JNK-1 is strictly regulated, and only the phosphorylated form of JNK (phospho-JNK) which is translocated to the nucleus has an ability to activate c-Jun response. There is a protein which inhibits JNK-1 activation, and known as JNK interacting protein-1 (JIP-1). In this study, we investigated change in JNK-1, phospho-JNK, and JIP-1 immunoreactivity in rat brain after transient middle cerebral artery (MCA) occlusion. Immunoreactive JNK-1 was scant in the sham-control brain, but it was induced at 1 h after reperfusion, which was slightly increased at 3 h of reperfusion. By contrast, phospho-JNK remained negative till 3 h. At 8 h, JNK-1 and phospho-JNK became distinctly positive, and nuclei as well as cytoplasm were stained. Thereafter, immunoreactivity for JNK-1 and phospho-JNK became furthermore dense, and most neurons revealed positively stained nuclei. Immunoreactivity for JIP-1 remained negative till 8 h of reperfusion, but at 24 and 72 h, cytoplasm of cortical neurons at the MCA boundary area was positively stained. This JIP-1 induction got behind the JNK-1 activation, and therefore, may be a vain effort for neurons to survive. Inhibition of JNK-1 activation might become an innovative means of therapy for stroke treatment in the future.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Stroke  ISOMapk8 (Rattus norvegicus)1582317; 1582317 RGD 
Stroke  IDA 1582317 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mapk8  (mitogen-activated protein kinase 8)

Genes (Mus musculus)
Mapk8  (mitogen-activated protein kinase 8)

Genes (Homo sapiens)
MAPK8  (mitogen-activated protein kinase 8)

Objects referenced in this article
Gene Mapk8ip1 mitogen-activated protein kinase 8 interacting protein 1 Rattus norvegicus

Additional Information