RGD Reference Report - Local signaling by the EGF receptor. - Rat Genome Database

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Local signaling by the EGF receptor.

Authors: Kempiak, SJ  Yip, SC  Backer, JM  Segall, JE 
Citation: Kempiak SJ, etal., J Cell Biol. 2003 Sep 1;162(5):781-7.
RGD ID: 1581920
Pubmed: PMID:12952932   (View Abstract at PubMed)
PMCID: PMC2172819   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200303144   (Journal Full-text)

Differing spatial scales of signaling cascades are critical for cell orientation during chemotactic responses. We used biotin EGF bound to streptavidin-coupled magnetic beads to locally stimulate cells overexpressing the EGF receptor. We have found that EGF-induced actin polymerization remains localized even under conditions of receptor overexpression. Conversely, EGF-induced ERK activation spreads throughout the cell body after EGF bead stimulation. The localized actin polymerization is independent of PI3-kinase and rho protein activity and requires Arp2/3 complex and cofilin function. Thus, we find differing spatial scales of signaling from the EGF receptor, supporting models of chemotaxis that integrate short- and long-range signaling.

Objects referenced in this article
Gene Egfr epidermal growth factor receptor Rattus norvegicus

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