RGD Reference Report - Regulation of dishevelled and beta-catenin in rat skeletal muscle: an alternative exercise-induced GSK-3beta signaling pathway. - Rat Genome Database

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Regulation of dishevelled and beta-catenin in rat skeletal muscle: an alternative exercise-induced GSK-3beta signaling pathway.

Authors: Aschenbach, WG  Ho, RC  Sakamoto, K  Fujii, N  Li, Y  Kim, YB  Hirshman, MF  Goodyear, LJ 
Citation: Aschenbach WG, etal., Am J Physiol Endocrinol Metab. 2006 Jul;291(1):E152-8. Epub 2006 Feb 14.
RGD ID: 1581696
Pubmed: PMID:16478782   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpendo.00180.2005   (Journal Full-text)

beta-catenin is a multifunctional protein involved in cell-cell adhesion and the Wnt signaling pathway. beta-Catenin is activated upon its dephosphorylation, an event triggered by Dishevelled (Dvl)-mediated phosphorylation and deactivation of glycogen synthase kinase-3beta (GSK-3beta). In skeletal muscle, both insulin and exercise decrease GSK-3beta activity, and we tested the hypothesis that these two stimuli regulate beta-catenin. Immunoblotting demonstrated that Dvl, Axin, GSK-3beta, and beta-catenin proteins are expressed in rat red and white gastrocnemius muscles. Treadmill running exercise in vivo significantly decreased beta-catenin phosphorylation in both muscle types, with complete dephosphorylation being elicited by maximal exercise. beta-Catenin dephosphorylation was intensity dependent, as dephosphorylation was highly correlated with muscle glycogen depletion during exercise (r(2) = 0.84, P < 0.001). beta-Catenin dephosphorylation was accompanied by increases in GSK-3beta Ser(9) phosphorylation and Dvl-GSK-3beta association. In contrast to exercise, maximal insulin treatment (1 U/kg body wt) had no effect on skeletal muscle beta-catenin phosphorylation or Dvl-GSK-3beta interaction. In conclusion, exercise in vivo, but not insulin, increases the association between Dvl and GSK-3beta in skeletal muscle, an event paralleled by beta-catenin dephosphorylation.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein kinase binding  IPIGsk3b (Rattus norvegicus)1581696 RGD 
protein kinase binding  IPIDvl1 (Rattus norvegicus)1581696 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Objects Annotated

Genes (Rattus norvegicus)
Ctnnb1  (catenin beta 1)
Dvl1  (dishevelled segment polarity protein 1)
Gsk3b  (glycogen synthase kinase 3 beta)

Genes (Mus musculus)
Ctnnb1  (catenin beta 1)
Dvl1  (dishevelled segment polarity protein 1)
Gsk3b  (glycogen synthase kinase 3 beta)

Genes (Homo sapiens)
CTNNB1  (catenin beta 1)
DVL1  (dishevelled segment polarity protein 1)
GSK3B  (glycogen synthase kinase 3 beta)


Additional Information